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EC number: 222-657-4 | CAS number: 3567-69-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- Study was conducted to access the effect of test chemical Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was highly soluble in water. In-house solubility was found to be 50 g/L. Therefore 1 liter of stock solution was prepared per the concentration of 1g/L, and was kept for 1hr stirring. After this the stock was taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.16 cm
- Weight at study initiation (mean and range, SD): average0.4695 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 168.3 mg of CaCO3
- Test temperature:
- 23.5 °C
- pH:
- 7.28-7.53
- Dissolved oxygen:
- 8.11 mg/l
- Nominal and measured concentrations:
- 6.25mg/L,12.5mg/L,25mg/L,50mg/L or 100mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod: -----------------
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: no mortality was observed
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: no mortality was observed
- Details on results:
- no mortality was observed after 24, 48, 72 and 96 hours at LC0 and LC50 of 100 mg/L.
- Sublethal observations / clinical signs:
(In each test, result varies always check)
8.1. Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3 h
6 h
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.86
7.88
8.07
8.08
8.50
8.50
8.16
8.06
6.25
4
4
8.11
8.14
8.13
8.14
8.27
8.29
8.12
8.15
12.5
4
4
8.14
8.15
8.18
8.20
8.20
8.23
8.12
8.14
25
4
4
8.21
8.19
8.20
8.21
8.24
8.25
8.08
8.12
50
4
4
8.17
8.15
8.21
8.16
8.26
8.26
8.14
8.15
100
4
4
8.15
8.18
8.13
8.20
8.18
8.24
8.16
8.1
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
8.2
8.2
8.5
8.5
8.4
8.2
8.2
8.2
6.25
4
4
8.1
8.1
8.2
8.2
8.2
8.2
8.3
8.3
12.5
4
4
8.2
8.1
8.2
8.2
8.2
8.2
8.3
8.3
25
4
4
8.1
8.1
8.2
8.2
8.1
8.1
8.2
8.1
50
4
4
8.1
8.0
8.1
8.1
8.2
8.2
8.1
8.1
100
4
4
8.0
7.9
8.1
8.1
8.2
8.2
8.0
8.0
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24 h
48 h
72 h
96 h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
23.9
23.9
21.7
21.7
22.0
22.0
23.1
23.1
6.25
4
4
24.0
24.0
21.9
21.9
22.0
22.1
23.2
23.2
12.5
4
4
24.0
24.0
21.9
21.9
22.2
22.2
23.1
23.2
25
4
4
24.2
24.2
21.9
22.0
22.2
22.1
23.1
23.1
50
4
4
24.4
24.5
22.1
22.0
22.2
22.2
23.1
23.1
100
4
4
24.5
24.6
22.0
22.0
22.1
22.1
23.2
23.2
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) on Zebra Fish Danio rerio was determine to be >100mg/L.
- Executive summary:
Study was conducted to assess the effect of test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
The test substance was highly soluble in water. In-house solubility was found to be 50 g/L. Therefore 1 liter of stock solution was prepared per the concentration of 1g/L, and was kept for 1hr stirring. After this the stock was taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
Reference
Description of key information
After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Study was conducted to assess the effect of test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
The test substance was highly soluble in water. In-house solubility was found to be 50 g/L. Therefore 1 liter of stock solution was prepared per the concentration of 1g/L, and was kept for 1hr stirring. After this the stock was taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
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