Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Principles of method if other than guideline:

Adsorption coefficient of test chemical was determined by using HPLC method.

GLP compliance:

no

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

25°C

Details on study design: HPLC method:

HPLC Procedure:
The solutions of the test substance and reference substances were prepared in solvent mentioned in 4.3. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k(table 3).The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor.

Operating Conditions:
HPLC Model No. : Agilent Technologies,1260 Infinity
Flow Rate : 1.0ml/min
Column : ZORBAX Eclipse Plus C18, 4.6×100mm, 3.5 μm
Mobile phase : ACN : water (55:45)
Temperature : 25°C
Detector : Diode Array Detector @210nm
Injector : Autosampler
Injector volume : 10μl
Data Acquisition software : ChemStationOpen lab Control Panel

Analytical monitoring:

not specified

Key result

Sample No.:

#1

Type:

log Koc

Value:

3.313 dimensionless

Temp.:

25 °C

Remarks on result:

other: other details not specified

Details on results (HPLC method):

The Adsorption coefficient (KOC) on soil and on sewage sludge of the test chemical was determined to be 3.313 ± 0.007 at 25°C

Transformation products:

not specified

Table 1:

Reference substance	Log Koc	tR1	tR2	tR3	MeantR± S.D.	k	Log k
Urea	-	0.887	0.887	0.888	0.887	-	-
Phenol	1.32	1.455	1.455		1.455	0.640	-0.193
Toluene	2.369	4.378	4.382		4.38	3.938	0.595
Xylene	2.58	6.241	6.232		6.2365	6.031	0.78
Ethylbenzene	2.649	6.359	6.355		6.357	6.167	0.79
Naphthalene	2.75	6.127	6.218		6.1725	5.959	0.775
phenanthrene	4.09	11.996	11.779		11.8875	12.402	1.093

Table 2:

Test substance Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate		K	Log k	Log Koc by Generalized Calibration Graph	Mean of LogKoc± S.D.
tR1	0.8	0.098	1.009	3.308	3.313 ± 0.007
tR2	0.801	0.097	1.014	3.317349

Validity criteria fulfilled:

yes

Conclusions:

The Adsorption Coefficient of test substance Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 3.313 ± 0.007 at 25°C. Thus based on the result it is concluded that the test substance has a strong sorption to soil and sediment and therefore has negligible to slow migration potential to ground water

Executive summary:

The adsorption coefficient Koc in soil and in sewage sludge Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference substances were chosen according to structural similarity with the test substance and calibration graph was prepared. The reference substances were Phenol, Toluene, Xylene, Ethylbenzene, Naphthalene, Phenenthrene having K_ocvalue ranging from 1.32 to 4.09 TheLog K_ocvalue was determined to be3.313 ± 0.007at 25°C.Thus based on the result it is concluded that the test substance has a strong sorption to soil and sediment and therefore has negligibleto slowmigration potential to ground water.

Additional information

Various experimental data for the target compound were reviewed for the Adsorption end point which are summarized as below:

 

The adsorption coefficient Koc in soil and in sewage sludgeDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference substances were chosen according to structural similarity with the test substance and calibration graph was prepared. The reference substances were Phenol, Toluene, Xylene, Ethylbenzene, Naphthalene, Phenenthrene having K_ocvalue ranging from 1.32 to 4.09 TheLog K_ocvalue was determined to be 3.313 ± 0.007at 25°C.Thus based on the result it is concluded that the test substance has a strong sorption to soil and sediment and therefore has negligibleto slowmigration potential to ground water.

 

The adsorption coefficient of chemical acid red 14 was studied by using Freundlich adsorption isotherm. The aim of the investigation is to determine the adsorption behavior of azo dyes in aqueous solution on DG06, GSE17200, and GSE17201 soils using C. I. Acid Red 14. GSE17200 and GSE17201 soils were obtained from Bayer Landwirtschaftszentrum, Monheim, Germany. DG06 soil was sampled in the surface layers (0– 20 cm) located in Dalian, Liaoning Province, China. Three kinds of soils were air-dried, sieved to 2 mm and stored in dark at a room temperature (20 ±1°C). The effect of initial dye concentration on the dye adsorption capacity was investigated between 100 and 800 mg/L at 20 ± 0.5°C. The dye uptake efficiencies of AR14 on DG06, GSE17200, and GSE17201 soils are high, and they are 21.9%, 10.4%, and 8.0%, respectively. By using Freundlich adsorption isotherm, Kf(Freundlich Constants)values were found to be 7.1*10-3 L/mg, 4.8*10-3L/mg, and 3.2*10-3L/mg forDG06, GSE17200, and GSE17201 soils respectively indicating high adsorption capacity of chemical Acid red 14.

On the basis of above results for target chemical, it can be concluded that the test substanceDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)has a strong sorption to soil and sediment and therefore has negligibleto slowmigration potential to ground water.