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EC number: 222-657-4 | CAS number: 3567-69-9
Endpoint summary
Administrative data
Description of key information
Short term toxicity to fish
Study was conducted to assess the effect of test chemical Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
The test substance was highly soluble in water. In-house solubility was found to be 50 g/L. Therefore 1 liter of stock solution was prepared per the concentration of 1g/L, and was kept for 1hr stirring. After this the stock was taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
Short-term toxicity to aquatic invertebrates
Determination of the inhibition of the mobility of daphnids was carried out with the substanceDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)according to OECD Guideline 202.
The test substance was tested at the concentrations 0, 10, 45,67, 100 and 150 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 150 mg/l was prepared by dissolving dark red powder in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water.
Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance, Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) in Daphnia magna was determined to be 203.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be non-hazardous to aquatic invertebrate and cannot classified as per the CLP classification criteria.
Toxicity to aquatic algae and cyanobacteria
Various experimental data for the target compound were reviewed for to assess the effects of test chemical Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) on the growth of fresh water green alga along with the predicted data which are summarized as below:
The Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Disodium 4-hydroxy- 3-[(4-sulphonatonaphthyl) azo]naphthalenesulphonate according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 2.6, 5.6, 12.0, 27.0, 59.0 and 130 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate, in Desmodesmus subspicatus was determined to be 265.1 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl) azo] naphthalenesulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus).
Further, the effect of test item Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate, CAS No. 3567-69-9 was studied on the growth of fresh water green alga Chlorella vulgaris (SSS, 2016).The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 34.823 mg/l.
According to Estimated 72 hrs EC50 value of test substance C. I. Acid Red 14 on Pseudokirchneriella subcapitata was determined by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database. Based on inhibition in growth rate of test organism Pseudokirchneriella subcapitata, the estimated 72 hrs EC50 value was determined to be 216.2179 mg/l.
As per the CLP classification, the test material is likely to be classified for aquatic classification category Chronic 3.
But as per CLP criteria, since the chemical is readily biodegradable and so the chemical is not considered to be hazardous.
Additional information
Short term toxicity to fish
Study was conducted to assess the effect of test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
The test substance was highly soluble in water. In-house solubility was found to be 50 g/L. Therefore 1 liter of stock solution was prepared per the concentration of 1g/L, and was kept for 1hr stirring. After this the stock was taken for experiment. From this stock solution further test concentrations were prepared for achieving test concentrations of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L, 100 mg/L respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.
After 96 hours of exposure of test chemical at of 100mg/L, no mortality was observed. Therefore the LC50 was determined to be >100mg/L. Based on the LC50, the test chemicalDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)can be considered as non-toxic and can be concluded as not classified as per the CLP classification criteria.
Short-term toxicity to aquatic invertebrates
Determination of the inhibition of the mobility of daphnids was carried out with the substanceDisodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate(CAS No.3567-69-9)according to OECD Guideline 202.
The test substance was tested at the concentrations 0, 10, 45,67, 100 and 150 mg/L. Effects on immobilisation were observed for 48 hours. The stock solution 150 mg/l was prepared by dissolving dark red powder in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water.
Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (EC50) for the test substance, Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) in Daphnia magna was determined to be 203.2 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be non-hazardous to aquatic invertebrate and cannot classified as per the CLP classification criteria.
Toxicity to aquatic algae and cyanobacteria
Various experimental data for the target compound were reviewed for to assess the effects of test chemical Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate (CAS No. 3567-69-9) on the growth of fresh water green alga along with the predicted data which are summarized as below:
The Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Disodium 4-hydroxy- 3-[(4-sulphonatonaphthyl) azo]naphthalenesulphonate according to OECD Guideline 201. The test substance was dissolved in OECD growth medium and tested at the concentrations 0, 2.6, 5.6, 12.0, 27.0, 59.0 and 130 mg/L. Effects on the growth rate of the organism were studied. The median effective concentration (ErC50) for the test substance, Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalenesulphonate, in Desmodesmus subspicatus was determined to be 265.1 mg/L. Based on this ErC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl) azo] naphthalenesulphonate does not exhibit toxicity to aquatic algae (Desmodesmus subspicatus).
Further, the effect of test item Disodium 4-hydroxy-3-[(4-sulphonatonaphthyl)azo]naphthalene sulphonate, CAS No. 3567-69-9 was studied on the growth of fresh water green alga Chlorella vulgaris (SSS, 2016).The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 34.823 mg/l.
According to Estimated 72 hrs EC50 value of test substance C. I. Acid Red 14 on Pseudokirchneriella subcapitata was determined by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database. Based on inhibition in growth rate of test organism Pseudokirchneriella subcapitata, the estimated 72 hrs EC50 value was determined to be 216.2179 mg/l.
As per the CLP classification, the test material is likely to be classified for aquatic classification category Chronic 3.
But as per CLP criteria, since the chemical is readily biodegradable and so the chemical is not considered to be hazardous.
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