Ethyl chloroformate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Due to lower dokumentation requirements in times before GLP the study is reliable with restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl chloroformate

EC Number:

208-778-5

EC Name:

Ethyl chloroformate

Cas Number:

541-41-3

Molecular formula:

C3H5ClO2

IUPAC Name:

ethyl chlorocarbonate

Details on test material:

- Name of test material (as cited in study report): Aethylchlorkohlensäureester (ethy carbonochloric acid ester)
- Substance type:
- Physical state: liquid
- Analytical purity: ca. 95 %
- internalTest substance No. XIX/351.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner
- Weight at study initiation: 216,9 +/- 27.7 (males); 166,8 +/-15,3 (females
- Fasting period before study: no data (probably: yes)
- Housing: no data (probably: grousp of 5)
- Diet (e.g. ad libitum): no data (probably: ad libitum)
- Water (e.g. ad libitum): no data (probably: ad libitum)
- Acclimation period:no data


ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Vehicle aqueous solution with 0.1 -1 % traganth gum
- Concentration in vehicle: 1 %

MAXIMUM DOSE VOLUME APPLIED: ca. 10 ml

Doses:

80, 100, 125, 160, 200,250, 320, 400 µl/kg

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were several times the day of application and at least daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

none

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 205 mg/kg bw

Remarks on result:

other: desity 1,14 g/cm3

Mortality:

400 µl/kg: 10/10 male and 10/10 female (within the first 24 h)
320 µl/kg: 10/10 male and 10/10 female (with the exeption of one male all within the first 24 h)
250 µl/kg: 5/10 male and 10/10 female (all death occurred within the first 48 h)
200 µl/kg: 5/10 male and 8/10 female
160 µl/kg: 2/10 male and 1/10 female
125 µl/kg: 0/10 male and 6/10 female
100 µl/kg: 0/10 male and 6/10 female
80 µl/kg: 0/10 male and 2/10 female

Clinical signs:

Dyspnea; apathy; staggering; abdominal and lateral position; sometimes twitching

Body weight:

not determined at the end of the study

Gross pathology:

Animals founfd dead: Dark-red discoloration of the gastric mucosa; reddish intestinal contents; serous membranes rich in liquids. 2 x lung edema
Sacrificed animals: nothing abnormal

Any other information on results incl. tables

LD 50 was estimated to be 180 µL/kg. this is equivalent to 205 mg/kg bw based on a density of 1.14 g/cm³ at 20°C

Signs of toxicity: dyspnea; apathy; staggering; abdominal and lateral position; sometimes twitching.

Necropsy findings: dark-red discoloration of the gastric mucosa; reddish intestinal contents; serous membranes rich in liquids.