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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
dermal absorption in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: non glp non-guideline study from publication

Data source

Reference
Reference Type:
publication
Title:
Comparative toxic effect of croton and butyric aldehydes
Author:
Trofimova
Year:
1960
Bibliographic source:
gif tr prof zabol

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
mouse tail immersion
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Crotonaldehyde
EC Number:
224-030-0
EC Name:
Crotonaldehyde
Cas Number:
4170-30-3
Molecular formula:
C4H6O
IUPAC Name:
but-2-enal
Details on test material:
colorless
specific gravity: 0.859
Molecular weight: 70.09
boiling point: 104-106 C
solubility in water: 18% at 20C
Radiolabelling:
no

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Type of coverage:
open
Vehicle:
unchanged (no vehicle)
Duration of exposure:
15, 30, 60 and 120 min
Doses:
no data
No. of animals per group:
50
Control animals:
yes
Remarks:
physiological solution

Results and discussion

Conversion factor human vs. animal skin:
no data

Any other information on results incl. tables

At 15 -30 min exposure to crotonaldehyde, 8 -12 mice died in the experiment and in the first day. At 1 -2 hours, all 13 mice died in the experiment of in the first day.

After 15 -30 min of exposure to crotonaldehyde, mice showed slowing of respiration, slight paresis of the paws, disruption of motor coordination. The symptoms of the absorptive effect were more pronounced after 2 -1 hrs of exposure: laying on the side, weakening of reflexes, paresis of the extremities, slowing of respiration, rigidity of the tail. In the mice that were still alive at the end of the experiment, an inflammatory process of the tail developed, which ended in necrosis and complete or partial rejection of dead portions of the tail.

The macroscopic examination of the organs of the dead mice showed plethora and the odor of aldehydes from the internal organs. The morphological examination of the organs of the mice that died in the experiment or that were killed on the 5th day of the experiment showed significantly expressed vascular disorders in the form of a plethora of internal organs, signs of plasmostasis, fine hemorrhages in the liver, kidneys and myocardium, regions of serous edema in the lungs. Sometimes there were signs of vasculitis, peribronchitis and fine regions of perobronchial interstitial pneumonia in the lungs. Small cell accumualtion and edema of perivascular zones were seen in the kidneys, liver and myocardium.

Applicant's summary and conclusion

Conclusions:
crotonaldehyde is absorbed through the skin in lethal amounts in mice
Executive summary:

crotonaldehyde is absorbed through the skin in lethal amounts in mice