Crotonaldehyde

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: publication

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

no ecoli strains

Principles of method if other than guideline:

Rannug et al (1976) modified liquid suspension

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Crotonaldehyde

EC Number:

224-030-0

EC Name:

Crotonaldehyde

Cas Number:

4170-30-3

Molecular formula:

C4H6O

IUPAC Name:

but-2-enal

Details on test material:

Obtained from Merck, Darmstadt
98% purity
commercial product was further purified by distillation in vacuo to a purity of > 99.9% as confirmed by gas-liquid chromatography

Method

Target gene:

histidine

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA98 adm TA 100

Details on mammalian cell type (if applicable):

not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

s9

Test concentrations with justification for top dose:

0.05–0.4 µl/ml

Details on test system and experimental conditions:

Preincubation test at pH 6.6 and 7.4

Results and discussion

Test results

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

positive

Remarks on result:

other: other: preincubation method

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

None

Applicant's summary and conclusion

Conclusions:

Postive for mutagenicity in strain TA 100

Executive summary:

This publication reported the outcome of crotonaldehyde in the AMES bacterial mutagenic assay by two different methods. The Plate incorporation test resulted in negative results in all strains (Salmonella TA 100, TA 98, TA 1538, TA 1537, and TA 1535) tested with and without metabolic activation. Preincubation method of crotonaldehyde resulted in positive mutagenicity in Salmonella test strain TA 100 with and without metabolic activation. All other tested strains were negative. The results indicate that crotonaldehyde is positive in the AMES assay for strain TA 100.