Dinaphtho[1,2,3-cd:3',2',1'-lm]perylene-5,10-dione, (9,10-dihydro-9,10-dioxo-1-anthracenyl)amino derivs.

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 September, 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

not specified

Principles of method if other than guideline:

With this test, the inhibitory concentration (IC50) of test substances is determined that leads to an inhibition of oxygen consumption of aerobic bacteria after 3 hours under defined environmental conditions.

GLP compliance:

no

Specific details on test material used for the study:

Test material: FAT 45082/D

Analytical monitoring:

not specified

Details on sampling:

None

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

activated sludge

Details on inoculum:

None

Test type:

other: suspension test

Water media type:

not specified

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

20 ± 2 °C

pH:

None

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

Test substance: 10, 32 and 100 mg/l
Reference substance: 10, 32 and 100 mg/l

Details on test conditions:

Refer "Any other information on materials and methods incl. tables"

Reference substance (positive control):

not specified

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

15.1, 23.6 and 15.1 % were the inhibition values recorded at test concentrations 10, 32 and 100 mg/l respectively. For reference substance, the inhibition values recorded were 12.5, 81.1 and 93.5 % at 10, 32 and 100 mg/l, respectively.

Results with reference substance (positive control):

3,5-Dichlorphenol: IC50 (3h) 19 mg/l

Reported statistics and error estimates:

None

Validity criteria fulfilled:

yes

Conclusions:

FAT 45082/E was concluded to have IC50 (3h) >100 mg/l.

Executive summary:

FAT 45082/D was evaluated for toxicity potential to bacterial population in a study conducted according to methodology that is equivalent to OECD Guideline 209.  The inhibition found at test concentrations of 10, 32 and 100 mg/l were 15.1, 23.6 and 15.1 %, respectively. For the reference substance, the inhibition values recorded were 12.5, 81.1 and 93.5 % at 10, 32 and 100 mg/l, respectively. Based on these findings, FAT 45082/E was concluded to have IC50 (3h) >100 mg/l.

Description of key information

The inhibitory concentration (IC50) in a 3-hour test for FAT 45082 was found to be greater than 100 mg/l. The same value was reported to be >400 mg/l in supporting studies.

Key value for chemical safety assessment

EC50 for microorganisms:

400 mg/L

Additional information

A key study was performed to determine the toxicity of FAT 45082 to micro-organisms.The inhibitory concentration (IC50) in a 3 hour test for FAT 45082 was found to be greater than 100 mg/L.

Other supporting studies were carried out which showed following results:

IC50 (3h): >100 mg/l

BST: >300 mg/l

BST: >400 mg/l

The values from key and supporting studies suggests that FAT 45082 has low toxicity to microorganisms.