esterification product of 2-hydroxypropane-1,3-diyl-bis(2-methylprop-2-enoate) and 3-hydroxypropane-1,2-diyl-bis(2-methylprop-2-enoate) and diphosphorus pentoxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 November 2001 - 30 January 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1224866-76-5

Cas Number:

1224866-76-5

IUPAC Name:

1224866-76-5

Details on test material:

- Name of test material (as cited in study report): Glypho
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: Stable
- Storage condition of test material: Refrigeration at ca. 4°C
- Other: bottles not to be filled > 90%, oxygen inhibits polymerisation

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 and 100 mg/L (nominal)
- Sampling method: Duplicate samples taken from control (0 mg/L) and 100 mg/L just prior to addition of daphnids (time 0). The 100 mg/L test vessels were pooled after test conclusion (time 48) before duplicate sampling. Only one of each control duplicate was analyzed for time 0.
- Sample storage conditions before analysis: Samples frozen at ca -20 °C until analysis

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 31 mg test substance was dissolved completely in 300 mL test water by ultrasonic treatment for 10 minutes and intense stirring for 3 hours at room temperature in the dark.
The test medium was prepared just before introduction of the daphnids (start of the test).
- Controls: Blank (no test substance) only.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Slight turbidity noted throughout test period

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna Straus
- Source: In-house stock of a clone originally obtained from University of Sheffield, UK
- Age at study initiation (mean and range, SD): 6-24 hours, not first brood progeny
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L as CaCO3

Test temperature:

21 - 22 °C

pH:

6.9 - 8.0

Dissolved oxygen:

8.0 - 8.3

Salinity:

NA

Nominal and measured concentrations:

Nominal: 0 and 100 mg/L
Measured:

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beakers filled with 50 mL test medium
- Aeration: No, medium aerated to oxygen saturation prior to test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two
- Biomass loading rate: < 1 daphnid / 2 mL test medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Analytical grade salts reconstituted in purified water.
Composition:
CaCI2 x 2H2O, 294.0 mg/L
MgSO4 X 7H2O, 123.0 mg/L
NaHCO3, 65.0 mg/L
KCI, 5.8 mg/L

- Alkalinity: 0.8 mmol
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark, 30 minute transition period
- Light intensity: Between 200 and 1200 Lux during light periods

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobility after 24 and 48 hours of exposure (immobility: no swimming within 15 seconds after gentle agitation of the test beaker)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test
- Range finding study: Yes, but results not reported. Test substance concentrations were stable in the rangefinding test.

Reference substance (positive control):

not specified

Remarks:

Potassium dichromate is tested as a positive control at least once a year to demonstrate satisfactory test conditions.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: No immobilized daphnids in controls or at 100 mg/L

Details on results:

- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: A slight turbidity was observed throughout the test period
- Effect concentrations exceeding solubility of substance in test medium: Water solubililty of test substance is 63 g/L. Analytical recovery of the test substance from the Daphnia test medium was 93% of nominal.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

<10% immobilization in controls, > 3 mg DO/L throughout testing period

Conclusions:

The 48-hour EC50 (immobilisation) of the test substance to Daphnia magna is >100 mg/L. No immobilisation was seen at this concentration

Executive summary:

Toxicity of the test substance to Daphnia magna was assessed according to OECD TG203 under GLP criteria. A slight turbidity was observed throughout the test period, however analytical recovery of the test substance was 93% of nominal. No immobilisation of controls or test subjects was observed. The 48-hour EC50 (immobilisation) of the test substance to Daphnia magna is >100 mg/L.

The test was conducted according to internationally accepted test guidelines and under GLP criteria. It is considered reliable without restriction and is suitable for Risk Assessment, Classification & Labelling, and PBT Assessment.