Registration Dossier
Registration Dossier
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EC number: 700-945-5 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The effect of the test material on the growth of Scenedesmus subspicatus has been investigated and gave ELR50 values of greater than 100 mg/l loading rate WAF. Correspondingly the No Observed Effect Loading Rate was 100 mg/l loading rate.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Methods
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No. 201, “Alga, Growth Inhibition Test” referenced as Method C.3 of Commission Directive 92/69/EEC.
Procedure
Following a preliminary range finding study,Scenedesmus subspicatuswas exposed to a Water Accommodated Fraction (WAF) of the test material at a loading rate of 100 mg/L (six replicate flasks) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1 degrees C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a Coulter Multisizer II Particle Counter.
Results
Exposure of Scenedesmus subspicatus to the test material gave ELR50 values of greater than 100 mg/L loading rate WAF and correspondingly the No Observed Effect Loading Rate was 100 mg/L loading rate WAF. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L loading rate WAF. Analysis of the test preparations at 0 hours showed the measured test concentrations to be 0.8% of nominal. Analysis of test preparations at 72 hours showed a decline to below the limit of quantitation of the analytical method. This decline is considered to be due to adsorption to algal cells. Given that the concentration of the test material in the 72 hour test samples was less than the limit of quantitation of the analytical method, and because the test item is a complex substance requiring use of the WAF approach, ELR50 values are based on nominal loading rates.
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