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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Adopted: 13 April 2004
Qualifier:
according to guideline
Guideline:
other: Testing Guidelines for Studies of Chemicals, Section 3.2, "Daphnia sp. acute immobilisation test"
Version / remarks:
Notification No. 2018-12, issued by the National Institute of Environmental Research, Korea on April 9, 2018
GLP compliance:
yes
Specific details on test material used for the study:
Lot No. 180705
Purity: 99.08%
Appearance: White crystal
Molecular Weight: 693.57 g/mol
Molecular Formula: C36H30N3O6P3
Storage condition: Room temperature
Test organisms (species):
Daphnia magna
Details on test organisms:
Supplier: Environmental Toxicology Lab, HTRC
Age: Less than 24 hours after birth
Environmental condition: The daphnia were bred in 1 L cylinder glass bath. The temperature of breeding water is maintained within the range of 18-22 oC and photo periods are 16 hours light and 8 hours darkness.
Feeding: Feeds were supplied once a day: Chlorella vulgaris.
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
The total hardness of the test water was 225 mg/L as CaCO3 and alkalinity was 36 mg/L.
Test temperature:
The mean of temperature was 20.4 oC (min. 20.0 oC ~max. 20.9 oC).
pH:
The mean of PH was 7.93 (min. 7.79 ~ max. 8.08)
Dissolved oxygen:
The mean of Dissoved Oxygen concentration (DO) was 7.97 mg/L (min. 7.26 mg/L ~max. 8.52 mg/L)
Nominal and measured concentrations:
Nominal concentration: 100 mg/L
Details on test conditions:
20 Daphnia aged less than 24 hours were divided into 4 groups of five animals each. 100 mL of test solution was filled up to the 125 mL of glass beaker. The photoperiod was 16 hours light : 8 hours darkness, and temperature of test water was 20±2 oC.
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
There was no immobility observed in any of the daphnia exposed to control group and 100.0 mg/L of test solution at 48 hours. No abnormal behaviors were observed in control and treatment groups during a limit test.


Validity criteria fulfilled:
yes
Remarks:
The mortality in the control did not exceed 10% at the end of the test.
Conclusions:
The 48 hours EL50 value of Phenoxycycloposphazene to Daphnia magna was determined to be higher than 100.0 mg/L, NOELR was 100.0 mg/L.The NOELR of Phenoxycycloposphazene to Daphnia magna was 100.0 mg/L at 48 hours.It is considered that toxic effect was not observed because it was test substance which is not dissoved that analysis result was below the LOQ. Determination of EL50 and NOELR was based on nominal concentration (loading rate).
Executive summary:

The Daphnia sp. acute immobilisation test of Phenoxycycloposphazene was studied using Daphnia magna for 48 hours under the semi-static system. The test was performed in control, 100.0 mg/L (notminal concentration) as a limit test. The analytical values of concentration were not detected. Therefore, the results were expressed by nominal concentration (loading rate).

The results were obtained as follows:

 Observation

time

 EL50(1)

(mg/L)

 95% Confidence limits

(mg/L)

  NOELR(2)
 24 hours > 100.0   -  100.0
 48 hours  >100.0  -  100.0

(1) Median effect loading rates, based on nominal concentration of active ingredient

(2) No Observable Effect Loading Rate

- No abnormal behaviors were observed in any of the test daphnia exposed to control and treatment group during the test.

- The mean of PH was 7.93 (min. 7.79 ~ max. 8.08). The mean of Dissoved Oxygen concentration (DO) was 7.97 mg/L (min. 7.26 mg/L ~max. 8.52 mg/L) and the percent of air saturation value was 89.9% (min. 82.5% ~max. 96.6%). The mean of temperature was 20.4 oC (min. 20.0 oC ~max. 20.9 oC).

The 48 hours EL50 value of Phenoxycycloposphazene to Daphnia magna was determined to be higher than 100.0 mg/L, NOELR was 100.0 mg/L. The EL50 and NOELR were based on nominal concentration (loading rate).

Description of key information

The 48 hours EL50 value was determined to be higher than 100.0 mg/L, NOELR was 100.0 mg/L (nominal concentration).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
100 mg/L

Additional information