Cuprate(4-), [C-(aminosulfonyl)-C-[[[2-[[4-chloro-6-[(2,5-disulfophenyl)amino]-1,3,5-triazin-2-yl]amino]ethyl]amino]sulfonyl]-29H,31H-phthalocyanine-C,C-disulfonato(6-)-N29,N30,N31,N32]-, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 - 26 May 1993 .

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD Guidelines for Testing of Chemicals (1984) No . 202 "Daphnia sp. Acute Immobilisation Test and Reproduction Test"

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/548/EEC).

Deviations:

not specified

GLP compliance:

not specified

Specific details on test material used for the study:

No further details specified in the study report.

Analytical monitoring:

yes

Details on sampling:

Verified by chemical analysis of the control and the 100 mg/l test groups at 0 and 48 hours

Vehicle:

no

Details on test solutions:

Direct dispersion in water. 200 mg of test material was dispersed in aged and dechlorinated tap water with the aid of ultrasonic disruption and the volume adjusted to 2 litres to give a 100 mg/l test concentration.

Test organisms (species):

Daphnia magna

Details on test organisms:

Name: Daphnia magna Straus.
Source: Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee, (I.R.CH.A}, France.
Culture: At 21 °C in polypropylene vessels containing two litres of aged and dechlorinated tap water. Cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.}. Culture conditions ensure that reproduction is by parthenogenesis.
Selection: Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

No post exposure observation period specified in the study report.

Hardness:

approximately 50 mg/l as Caco3.

Test temperature:

21 °C

pH:

7.7 - 7.8

Dissolved oxygen:

7.9 -8.2 mg O2/l

Salinity:

Not applicable-freshwater study

Conductivity:

Not specified

Nominal and measured concentrations:

Verification of test concentrations showed the measured concentrations from analysis at 0 and 48 hours to be near nominal. It was therefore considered justifiable to estimate the Ec 50 values based on nominal test concentrations only .

Details on test conditions:

Test vessels: Glass jars each containing 200 ml test solution and covered to reduce evaporation.
Experimental design: 1 test concentration of 4 replicates plus 1 control in duplicate. 10 animals per concentration.
This study design conforms to a Limit Test to show no effect on the test organisms at the highest recommended test concentration in the OECD/ EEC test guidelines.
Method of initiation: Daphnia were placed in the test solutions after addition of the test material.
Loading: 20 ml test solution per organism.
Photoperiod: 16 h light : 8 h dark.
Temperature: 21.0 °C
Aeration: None.
Test concentration: 100 mg/l, 4 replicates. This test concentration was assigned following a preliminary range-finding study where 10 daphnids were exposed for a period of 48 hours to each of the following test concentrations: 0.10, 1. 0, 10 and 100 mg/l. The results showed no effect at all the test concentrations thereby indicating the choice of test concentration employed in the test.
Medium renewal: None.
Duration of exposure: 48 hours.
Environmental Measurements: Temperature was recorded daily. pH and dissolved oxygen concentrations were recorded at initiation and termination of the study .
Criteria of effect: Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.

Inspection of the immobility data gave the following results based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.

The “No Observed Effect Concentration" is based upon zero immobilisation at this test concentration.
It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Results with reference substance (positive control):

Not specified

Cumulative Immobilisation Data for Daphnia magna Exposed for 48 Hours to JPR Blue 100

Nominal Concentration (mg/l)	Cumulative immobilized Daphnia (initial population: 10 per replicate)
	24 hours			48 hours
	No. per replicate	Total	%	No. per replicate	Total	%
Control           R1                       R2	0 0	0	0	0 0	0	0
100                 R1                       R2                       R3                       R4	0 0 0 0	0	0	0 0 0 0	0	0

R₁– R₄= Replicates 1 – 4

 

Environmental Measurements for Daphnia magna Exposed for 48 hours for JPR Blue 100

Nominal Concentration (mg/l)	0 hours			24 hours	48 hours
	pH	mg O2/l	T °C	T °C	pH	mg O2/l	T °C
Control           R1                       R2	7.8 7.8	8.2 8.2	21.0 21.0	21.0 21.0	7.7 7.7	7.9 7.9	21.0 21.0
100                  R1                        R2                        R3                        R4	7.8 7.8 7.8 7.8	8.2 8.2 8.2 8.2	21.0 21.0 21.0 21.0	21.0 21.0 21.0 21.0	7.7 7.7 7.7 7.7	7.9 7.9 7.9 7.9	21.0 21.0 21.0 21.0

R₁– R₄= Replicates 1 - 4

Validity criteria fulfilled:

not specified

Conclusions:

Inspection of the immobility data gave the following results based on nominal test concentrations:
Time (h) EC50 (mg/l)
24 >100
48 >100
"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.
"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.

Executive summary:

TEST SPECIES: Daphnia magna Straus tested in the 1st instar.

 

TEST TYPE: Acute toxicity. 48h EC50 (immobilisation).

 

TEST CONDIT IONS: Static test conditions according to OECD Guideline No. 202, referenced as Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC).

 

RESULTS

There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.

 

The following results are based on nominal test concentrations:

Time (h)        EC50 (mg/l)

24                  >100

48                  >100

"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.

"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.

 

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Description of key information

The following results are based on nominal test concentrations:

Time (h)        EC50 (mg/l)

24                  >100

48                  >100

"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

TEST SPECIES: Daphnia magna Straus tested in the 1st instar.

TEST TYPE: Acute toxicity. 48h EC50 (immobilisation).

TEST CONDIT IONS: Static test conditions according to OECD Guideline No. 202, referenced as Method C.2 of Commission Directive 84/ 449/ EEC (which constitutes Annex V of Council Directive 67/ 548/ EEC).

 

RESULTS

There were no immobilised Daphnia or other adverse reactions to exposure in 40 daphnids exposed to a test concentration of 100 mg/l for a period of 48 hours.

The following results are based on nominal test concentrations:

Time (h)        EC50 (mg/l)

24                  >100

48                  >100

"No Observed Effect Concentration” (NOEC) at 24h ≥ 100 mg/l.

"No Observed Effect Concentration” (NOEC) at 48h ≥ 100 mg/l.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.