N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-02-29 - 2012-03-03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Name of test material (as cited in study report): Bis-DMAPA
- Physical state: liquid
- Supplier: SGS Belgium N.V.
- Batch number: PFW110019
- Purity: 97.8%
- Purity test date: May 30th 2011
- Total amine content: 16.02 meq/g
- Storage condition of test material: lab storage

Analytical monitoring:

yes

Details on sampling:

- Samples for test concentration analysis were taken at 0 and 48 h from all test solutions (at the start of the test excess test solutions were sampled and at the end of the test 1 replicate of each test solution was sampled).
- Sampling method: not specified
- Sample storage conditions before analysis: not specified

Vehicle:

no

Details on test solutions:

- Controls: dilution water
- A stock concentrate of Bis-DMAPA with a nominal concentration of 120 mg/L was prepared by adding a nominal 0.24 g of test substance to 2000 mL of dilution water. The concentrate was inverted several times, which resulted in a clear and colourless solution. This stock concentrate was then used to prepare the test solutions. This was achieved by the direct addition of the appropriate amount of concentrate to 1000 mL of dilution water in a volumetric flask. For the highest exposure concentration the concentrate was used without any further dilution.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: diploid parthenogenesis
- Feeding during test: no

ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): reared in same conditions as test
- Type and amount of food: a mixed algae diet of Chlorella vulgaris, strain CCAP 211/12 and Pseudokirchneriella subcapitata, strain CCAP 278/4, supplied ad libitum
- Feeding frequency: depending on age and density of the culture
- Health during acclimation: test organisms and culture from which they were obtained showed no evidence of disease before the test period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

222.3 mg CaCO3/L

Test temperature:

20 ± 1°C

pH:

Start of test: 8.2-8.8
End of test: 7.8-8.3

Dissolved oxygen:

Start of test: 7.5-8.2 mg/L
End of test: 8.2-9.1 mg/L

Salinity:

N/A

Nominal and measured concentrations:

Nominal exposure concentrations: control, 5, 11, 25, 55, 120 mg/L.
The mean measured concentrations ranged from 100-117% of nominal.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): covered with loose fitting glass lid
- Material, size, headspace, fill volume: glass, 250 mL, 200 mL fill volume
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water medium - Elendt's M4 D. magna medium
- Total organic carbon: < 1 mg/L
- Particulate matter: < 3 mg/L
- Pesticides: < 0.1 µg/L
- Alkalinity: 39.6 mg CaCO3/L
- Conductivity: 608 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: by batch

OTHER TEST CONDITIONS
- Adjustment of pH: with 1M HCl to suitable range
- Photoperiod: 16 hours light:8 hours dark, with 20 minute transition periods
- Light intensity: normal ambient

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, 24 and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

50.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI = 41.1-63.7 mg/L

Details on results:

- Behavioral abnormalities: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none
- 24-h EC50 = 53.3 mg/L (95% CI = 43.4-67.9 mg/L)
- 48-h NOEC = 25 mg/L
- 48-h LOEC = 55 mg/L

Reported statistics and error estimates:

The 24 and 48 hour EC50s were calculated using the moving average angle method.

Validity criteria fulfilled:

yes

Conclusions:

In this study, performed according to OECD guideline 202, the 48-h EC50 of the test substance for the freshwater invertebrate Daphnia magna was calculated to be 50.3 mg/L. Test concentrations were verified at the start and end of testing and agreed with nominal concentrations. The results of this well performed study can be considered reliable.

Description of key information

One study (Fox et al., 2012) is considered as the key study for endpoint coverage. The study investigates the acute toxicity of the substance to Daphnia magna according to the OECD guideline 202 under static conditions. The 48h-EC50 was determined to be 50.3 mg test material/L (based on nominal concentrations). The study is given a Klimisch score of 1 and was conducted under GLP.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

50.3 mg/L