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EC number: 229-761-9 | CAS number: 6711-48-4
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, Part 15
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Bis- (3-dimethylaminopropyl)amin
- Analytical purity: 98.4 %
- Lot/batch No.: 94-0675
- Physical state: Liquid
- Stability: The stability of the test substance over the study period was proven by reanalysis
- Homogeneity: The test substance is homogeneous
- Solubility in water: Miscible up to 10 g/L
- Stability in water: The test substance was stable in the carrier for the period specified - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared separately for each test concentration. The substance was added to the test medium and stirred with an ultraturrax. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Charles River Aquatics, Someren; Netherlands
- Age at study initiation (mean and range, SD): 8 month and 3 month respectively
- Length at study initiation (length definition, mean, range and SD): 1) 3.30 cm (range 2.8 - 4.0 cm); 2) 2.85 cm (2.6 - 3.2 cm)
- Weight at study initiation (mean and range, SD): 1) 0.29 g (0.18 - 0.48 g); 2) 0.24 g (0.20 - 0.40 g)
ACCLIMATION
- Acclimation period: 14 d
- Type and amount of food: TETRA MIN standard feed for aquarium fish TETRA - WERKE, Melle, Germany, ad libitum; and on workdays generally additionally live artemia
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 1) no mortality observed 2) 4.3 % - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.55 mmol/l
- Test temperature:
- 21 - 24 °C
- pH:
- 8.3 - 10.2 (neutralised sample: 8.2)
- Dissolved oxygen:
- 7.6 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations (mg/L): 0 (control), 0 (control), 1.0, 2.15, 4.64, 10.0, 21.5, 46.4, 100, 215, 464, 1000, 1000 (neutralised)
Analytically determined concentrations (mg/L): < 1 mg/L (control), 1.3, 2.2, 4.7, 9.9, 21.2, 47, 100, 220, 470, 1000, 1030 - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.29 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the City of Frankenthal, not chlorinated and passed through a charcoal filter, aerated
- Total hardness: ca. 2.5 mmol/L
- Acid capacity: ca. 5.5 mmol/L
- Ca content: ca. 90 mg/L
- Mg content: ca. 10 mg/L
- pH: ca. 8.0 - 8.6
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Feeding: Withdrawal of food: 1 day before and during exposure
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC 50 (96 h): >100 and <1000 mg/L - Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 215 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 68 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: The LC50 was determined by calculating the geometric mean between the highest concentration causing no mortality and the lowest concentration producing 100% mortality.
- Details on results:
- Observed symptoms: apathy, tumbling, swimming near water surface
- Sublethal observations / clinical signs:
Measured pH-values:
Test item concentration (mg/l; nominal)
pH after (h)
1 h
24 h
48 h
72 h
96 h
0
8.3
8.3
8.3
8.3
8.3
0
8.4
8.5
8.6
8.6
8.6
1
8.3
8.2
8.2
8.3
8.3
2.15
8.4
8.3
8.2
8.3
8.3
4.64
8.4
8.4
8.3
8.3
8.3
10.0
8.5
8.4
8.4
8.4
8.5
21.5
8.7
8.4
8.4
8.5
8.5
46.4
8.8
8.6
8.6
8.6
8.7
100
9.1
8.7
8.7
8.6
8.7
215
9.3
9.1
464
9.7
1000
10.2
1000*
8.2
* pH-adjusted
Mortality:
Test item concentration (mg/l; nominal)
Dead fish after (h)
1 h
4 h
24 h
48 h
72 h
96 h
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
2.15
0
0
0
0
0
0
4.64
0
0
0
0
0
0
10.0
0
0
0
0
0
0
21.5
0
0
0
0
0
0
46.4
0
0
1
5
7
8
100
0
0
0
6
7
7
215
0
0
10
10
10
10
464
0
10
10
10
10
10
1000
10
10
10
10
10
10
1000*
0
10
10
10
10
10
* pH-adjusted
- Conclusions:
- The lowest LC50 value from the range resulting from the key study (21.4 mg/L) is taken as a worst case approach for further assessment.
Reference
Description of key information
One study (BASF, 1998), investigating the acute toxicity of the substance to fish according to the German Industrial Standard DIN 38412 part 15 (similar to OECD guideline 203), was considered as the key study for endpoint coverage. The 96h-LC50 was determined to be 21.4 mg test material/L (measured) based on mortality of the test species Danio rerio. The study is given a Klimisch score of 1 and was conducted under GLP.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 21.4 mg/L
Additional information
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