4-(1,1-dimethylethyl)cyclohexyl acrylate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1981-07-14 to 1981-10-15

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented, scientifically acceptable study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Principles of method if other than guideline:

according to BASF-internal standard

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-(1,1-dimethylethyl)cyclohexyl acrylate

EC Number:

282-104-8

EC Name:

4-(1,1-dimethylethyl)cyclohexyl acrylate

Cas Number:

84100-23-2

Molecular formula:

C13H22O2

IUPAC Name:

4-tert-butylcyclohexyl prop-2-enoate

Test material form:

liquid

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River WIGA, Sulzfeld, FRG
- Age at study initiation: about 4 weeks
- Weight at study initiation: males: 22 - 28 g; females: 22 - 24 g
- Fasting period before study: The animals are given no feed for 16 hours before administration, but water is available ad libitum.
- Housing: 5 animals per cage (Makrolon cages, type I)
- Diet: ad libitum (SSNIFF R; Ssniff Versuchstierdiäten, Soest, FRG)
- Water: ad libitum
- Acclimation period: at least one week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): 45 - 75
- Photoperiod (hrs dark / hrs light): 12/12 (6.00 - 18.00 hours/ 18.00 - 6.00 hours)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

olive oil

Details on exposure:

The test substance is insoluble in water.
Form of administration: emulsion
Concentrations used: 14, 20, 29.4 and 40 % (G/V)
Application volume: 5 mL/kg

Doses:

700, 1000, 1470 and 2000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: before administration, 2nd weighing 3 days, 3rd weighing 4 days, 4th weighing 7 days and 5th weighing 13 days after administration.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Cageside observation: Recording of signs and symptoms < 15 min, 15 min, 30 min, 1 h, 2 h, 4 h, and 5 h after administration of test substance and then once each workday. Check for moribund and dead animals twice each workday and once daily at weekends and on public holidays.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 - < 1 470 mg/kg bw

Mortality:

Male animals: 700 and 1000 mg/kg: no deaths after 14 days; 1470 and 2000 mg/kg: 5/5 after 14 days
Female animals: 700 and 1000 mg/kg: no deaths after 14 days; 1470 and 2000 mg/kg: 4/5 after 14 days

Clinical signs:

Male animals: Dyspnea, apathy, staggering, trembling, twitching, spastic gait, tonus with stretching, rolling convulsions, piloerection, exsiccosis, ptosis, disequilibrium, poor general state
Female animals: dyspnea, apathy, abnormal position, staggering, trembling, spastic gait, tonus with stretching, rolling convulsions, clonic convulsion, piloerection, exsiccosis, disequilibrium, paresis, ptosis, poor general state, cachexia

Body weight:

Mean body weight male animals: 25.5 g at study start, 33.2 g after 13 days
Mean body weight female animals: 23.0 g at study start, 27.5 g after 13 days

Gross pathology:

Animals that died: No intra abdominal precipitates of the test substance or adhesions.
Sacrificed animals: Organs: No abnormalities detected.