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EC number: 282-104-8 | CAS number: 84100-23-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
In an alga, growth inhibition test according to OECD guideline 201, recalculated EC50 - and EC10 -values for inhibition of specific growth rate after 72 h were 0.539 mg/L and 0.414 mg/L (based in time weighted average mean measured concentrations; BASF, 60E0580/09X174, 2015)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.539 mg/L
- EC10 or NOEC for freshwater algae:
- 0.414 mg/L
Additional information
An algae growth inhibition test with the
freshwater green algae Pseudokirchneriella subcapitata was conducted
according to OECD guideline 201 (BASF, 60E0580/09X174, 2015). The aim of
the study was to assess the effects on the growth rate over a period of
72 hours. The study was conducted under static conditions with an
initial cell density of 6153 x 10^3 cells/ mL. The green algae were
exposed to the nominal test concentrations of 1, 3.16, 10, 31.6 and 100
mg test item/L, corresponding to the time weighted average mean measured
concentrations of 0.428, 1.32, 2.85, 3.49 and 3.89 mg/L.
All test concentrations of the test substance and the control were
analytically verified by GC-MS of the two diastereomers after 24 hours
and at the end of the exposure. The measured concentrations of the test
substance at the start of the exposure were in the range of 3 to 34 % of
the nominal test item concentrations. After 24 hours, 4 to 41 % of the
nominal test item concentration were found. At the end of the exposure 4
to 55 % of the nominal test item concentration were measured. In all
loadings the measured concentration increased during the exposure time.
It is assumed that at the beginning of the test the test item was not
completely dissolved.
The original report states EC50 - and EC10 -values for inhibition of specific growth rate of 0.690 mg/L and <0.428 mg/L after 72 h of exposure
(based on time weighted average mean measured concentrations). As a "lower than"-value for an EC10 is not appropriate in a regulatory context, a recalculation based on the raw data given in the report (measured cell densities) was performed. This re-evaluation using the statistical software ToxRatPro Version 2.10 resulted in values for ErC50 and ErC10 of 0.539 mg/L and 0.414 mg/L, respectively. These values are used in the further assessment of the registration item.
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