Registration Dossier
Registration Dossier
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EC number: 256-181-3 | CAS number: 45021-77-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16/06/1996-21/06/1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented and scientifically acceptable report.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- The growth characteristics of the bacteria were not measured by turbidimetr by using a microbiological method with Petri dish cultures according to Bringmann and Kühn, 1977.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trimethyl-3-[(1-oxoallyl)amino]propylammonium chloride
- EC Number:
- 256-181-3
- EC Name:
- Trimethyl-3-[(1-oxoallyl)amino]propylammonium chloride
- Cas Number:
- 45021-77-0
- Molecular formula:
- C9H19N2O.Cl
- IUPAC Name:
- trimethyl-3-[(1-oxoallyl)amino]propylammonium chloride
- Details on test material:
- - Name of test material: DIMAPA Quat
- Physical state: liquid
- Analytical purity: 60%
- Composition of test material, percentage of components: 100%
- Lot/batch No.: not specified
- pH-value: 8
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Demineralised water
Test organisms
- Test organisms (species):
- Pseudomonas putida
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 5 d
Test conditions
- Nominal and measured concentrations:
- The nominal concentrations tested were 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000, 2000, 4000 mg/L.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 5 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 4 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- APTAC had no inhibitory effect on the growth of Pseudonomas putida at 4000 mg/l after 5 days.
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