Trimethyl-3-[(1-oxoallyl)amino]propylammonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diallyldimethylammonium chloride

EC Number:

230-993-8

EC Name:

Diallyldimethylammonium chloride

Cas Number:

7398-69-8

IUPAC Name:

N-allyl-N,N-dimethylprop-2-en-1-aminium chloride

Details on test material:

- Name of test material (as cited in study report): Dimethyldiallylammoniumchloride ca 60%
- Molecular formula (if other than submission substance): C8H16N.Cl
- Molecular weight (if other than submission substance): 161.68
- Smiles notation (if other than submission substance): C(=C)CN{+}(c)(c)(.Cl{-})CC=C
- InChl (if other than submission substance): 1/C8H16N.ClH/c1-5-7-9(3,4)8-6-2;/h5-6H,1-2,7-8H2,3-4H3;1H/q+1;/p-1
- Substance type: non-volatile, organic
- Physical state: colourless liquid
- Composition of test material, percentage of components: 60% aqueous solution of Dimethyldiallylammonium chloride
- Analytical purity: no data given
- Impurities (identity and concentrations): no data given
- Lot/batch No.: 76334
- Expiration date of the lot/batch: no data given
- Stability under test conditions: satable
- Storage condition of test material: room temperture

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge : BASF laboratory wastewater plants treating municipal sewage
- Concentration of sludge: 30mg/L

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

54 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: BASF laboratory wastewater plants treating municipal sewage:
- Test temperature: room temperature
- pH: 7.4-7.7
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 1000 ml vessels
- Number of culture flasks/concentration: 2 for test substance, 1 for the controls and the reference substance
- Method used to create aerobic conditions: no data
- Measuring equipment: no data
- Test performed in open system: no
SAMPLING
- Sampling frequency: Sample were taken for DOC analysis at days 0,1, 3, 5, 7, 10, 14, 17, 22, 24, 27, and 28
- Sampling method: no data
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Degradation degree in the inhibition control after 14days
- Abiotic sterile control: Physico-chemical (abiotic) elimination of the test substance
- Toxicity control: no data
- Other: Elimination of the test substance by absorption
At day 14 of the test 1ml mercury chloride was added to the abiotic control assay

Reference substance

Reference substance:

aniline

Results and discussion

Test performance:

Validity criteria: The test is valid
Deviation of the degradation degree of the test substance in the plateau phase < 20%
Degradation degree of the reference substance > 70% after 14 days.
Degradation degree in the inhibition control > 35% after 14 days

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

>= 40 - <= 50

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

ca. 0

Sampling time:

10 d

Details on results:

Degradation degree in the inhibition control after 14days: 40-50 %DOC
Physico-chemical (abiotic) elimination of the test substance: <10 % DOC
Elimination of the test subtance by absorption: <10 % DOC after 5 days

BOD5 / COD results

Results with reference substance:

Degradation % DOC 14 days = 90-100

Any other information on results incl. tables

DOC Decrease %

Day	RS	IH	PC	AC	TS1	TS2	TS Mean value
0	0	0	0	0	0	0	0
1	2	-1	-4	-4	-2	-2	-2
3	25	3	-12	-13	-6	-12	-9
5	94	46	-9	-10	-5	-6	-6
7	98	42	-17		-10	-12	-11
10	96	45	-9		-4	-9	-7
14	96	47	-9		0	-2	-1
17	93	49	-9		38	28	33
22	97	73	0		46	45	46
24	95	71	-9		43	40	42
27	99	70	-14		41	43	42
28	97	69	-13		45	43	44

RS = reference substance, IH = inhibition control, PC = abiotic control, AC = absorption control, TS = test substance

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Degradation of the test substance was 40-50% (but less than 70%) measured by DOC removal in 28 days. Consequently dimethyldiallylammonium chloride is not readily biodegradable according to the OECD criteria.