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EC number: 930-690-7
CAS number: -
OFFSPRING AND REPRODUCTION DATA
Analysis of Dunnet one-tailed test providing "no-observed-effect
concentration" (NOEC) values gave following results:
Summary Statistics and ANOVA
* - the mean for this group is significantly less than the control mean
at alpha = 0.05 (1- sided) by a
t– test with Bonferroni adjustment of alpha level
s.d. - standard deviation
n - number of parent animal
cv - coefficient of variation
The No Observed Effect Loading Rate (NOELR) after 21-day exposure was
0.10 mg/l. NOEC is based upon no significant change of reproduction for
exposed group compared to control group. The Lowest Observed Effect
Loading Rate (LOELR) after 21-day exposure was 0.21 mg/l.
Summary data analysed using software TOXEDO (5) providing EL50 values,
gave following results:
There was not observed reproduction toxicity more than 50% in daphnids
exposed to test substance WAF of all loading rates after 21 days. EL50
was greater than 5 mg/l. Time of first brood production was the 10th day
of test duration for all WAFs and control.
Complex data, for each concentration of test substance, for reproduction
toxicity with Daphnia magna are given in APPENDIX 1.
The test preparations were analysed for Total Organic Carbon (TOC).
Analysis of the new medium (at 0 hours) test preparations showed
measured test concentration (corrected for control) to range from 0.013
to 1.424 mg/l. Analysis of the old medium (after 48 or 72 hours) test
preparations showed measured test concentration (corrected for control)
to range from 0.055 to 1.719 mg/l with the one exception., 4.196 mg/l at
loading rate 0.10 mg/l. There was no significant decline in measured
test concentrations over 48 and 72 hours period in the highest test
Chemical analysis of TOC showed that measured concentrations in old
medium for ranged from 83% to 97% of nominal concentration of WAF at
loading rate 5 mg/l. In the WAFs with the lower loading rates, the TOC
concentration in new medium was
approximately in the same range as in the control vessel and in old
medium the TOC concentration was mostly higher than in control (this
consider due to feeding of animals). Thus, the measurements of TOC
originated form the test items was not possible due to low
test concentration and feeding of animals.
Given that toxicity cannot be attributed to a single component or
mixture of components but to the test material as a whole the results
were based on loading rates only.
Table 1: Daphnia Reproduction Data
Loading rate (mg/L)
Females alive at end of test
Offspring at termination
Offpsring per female
Inhibition in percent
In an 21-day Daphnia magna reproduction toxicity test according to OECD
211 and performed to GLP standard, daphnia were exposed to Shale oil
middle fraction in indivivdual vessels at nominal loading rates of 0.05,
0.10, 0.21, 0.47, 1.03, 2.27 and 5.00 mg/L.
The EL50 (Effective Loading Rate) of the test substance, Shale oil, in
the test of reproduction to Daphnia magna with 21-day exposure was
greater than 5 mg/l. The No Observed Effect Loading Rate (NOELR) after
21-day exposure was the concentration of WAF at loading rate 0.10 mg/l.
Based on the rationale for read-across, it is considered acceptable to
use this study to address the same endpoint for the heavy fraction of
The EL50 (Effective Loading Rate) of the test substance, Shale oil, in the test of reproduction to Daphnia magna with 21-day exposure was greater than 5 mg/l. The No Observed Effect Loading Rate (NOELR) after 21-day exposure was the concentration of WAF at loading rate 0.10 mg/l.
Similar values for short-term toxicity to daphnia were observed between
the middle fraction and heavy fraction of shale oil. This, combined with
analytical data which showed the two fractions to be compositionally
similar, support the validity of a read-across approach to address the
long-term toxicity to aquatic invertebrates endpoint. Hence, the study
conducted on the middle fraction of shale oil, is considered robust
enough to be the key study for the heavy fraction.
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