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EC number: 930-690-7
CAS number: -
Table 1: Immobilisation of Daphnia
Loading Rate (mg/l)
Number of Daphnids tested
Number immobilised after 24 hours
Number immobilised after 48 hours
EFFECTIVE INHIBITION LOADING RATES
Based on recorded inhibition of mobility in time, these effective
inhibition loading rates (EiL) were calculated:
24 – hour EiL10 = 7.36 mg/l
95 % confidence interval: 5.01 – 8.94 mg/l
24 – hour EiL50 = 11.57 mg/l
95 % confidence interval: 9.69 – 13.82 mg/l
48 - hour EiL10 = 5.89 mg/l
95 % confidence interval: 3.42 – 7.36 mg/l
48 - hour EiL50 = 9.71 mg/l
(95 % confidence interval: 8.34 – 11.33 mg/l)
Lowest loading rate being responsible for 100 % immobilization (EiL100)
After 24 – hours exposure = 50 mg/l (WAF loading rate)
After 48 – hours exposure = 25 mg/l (WAF loading rate)
Highest loading rate being responsible for 0 % immobilization (EiL0)
After 24 – hours exposure = 6.25 mg/l (WAF loading rate)
After 48 – hours exposure < 3.125 mg/l (WAF loading rate)
The aim of the study was to determine the loading rates of SHALE OILS,
HEAVY inhibiting the mobility of Daphnia magna in a 48-hour semi-static
immobilization test. The presented results are based on water
accommodated fractions (WAFs) and loading rates of the test substance
SHALE OILS, HEAVY, since the test substance belongs to complex
multi-component substances only partially soluble in water.
SHALE OILS, HEAVY was tested in loading rates 3.125; 6.25; 12.5; 25 and
50 mg/l. On the basis of the results, it is concluded that the test
substance immobilized 50 % of the test animals, the freshwater
crustacean Daphnia magna, at a loading rate 11.57 mg/l after 24 - hour
exposure (confidence limit: 9.69 – 13.82 mg/l) and at a loading rate
9.71 mg/l after 48 – hour exposure (confidence limit: 8.34 – 11.33 mg/l)
under semi-static conditions.
Also effective inhibition loading rates (EiL) were calculated for 10 %
immobilization of Daphnia magna in both time exposures: The 24 – hour
EiL10 was calculated on 7.36 mg/l (confidence limit: 5.01 – 8.94 mg/l)
and the 48 – hour EiL10 was calculated on 5.89 mg/l (confidence limit:
3.42 – 7.36 mg/l).
The lowest loading rate being responsible for 100 % immobilization
(EiL100) was determined after 24 – hour exposure at 50 mg/l and after 48
– hour exposure at 25 mg/l.
The highest loading rate being responsible for 0 % immobilization (EiL0)
was determined after 24 – hour exposure at 6.25 mg/l and after 48 – hour
exposure less than 3.125 mg/l.
The 48 hour LC50 to Daphnia magna was determined as 9.71 mg/l after 48 – hour exposure (confidence limit: 8.34 – 11.33 mg/l)
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