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EC number: 701-028-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- tris (4-nonylphenol, branch) phosphorous acid ester
- EC Number:
- 701-028-2
- Cas Number:
- 26523-78-4
- Molecular formula:
- C45H69O3P
- IUPAC Name:
- tris (4-nonylphenol, branch) phosphorous acid ester
- Reference substance name:
- Nonylphenol
- EC Number:
- 246-672-0
- EC Name:
- Nonylphenol
- Cas Number:
- 25154-52-3
- IUPAC Name:
- 2-nonylphenol
- Test material form:
- liquid
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, and Escherichia coli WP2 uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9
- Test concentrations with justification for top dose:
- 0, 75, 200, 600, 1800, 5000 ug/plate
- Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- yes
- Remarks:
- Acetone
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- Positive controls:
- yes
- Positive control substance:
- other: 1) all Salmonella strains and WP2 urvA with S9 : 2-aminoanthracene 2) TA98 without S9 : 2-nitrofluorene 3) TA100 and TA1535 without S9 : sodium azide 4) TA1537 without S9 : 9-amino-acridine 5) WP2 urvA without S9 : methyl methanesulfonate.
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS: Samples were run in triplicate, with and without metabolic activation.METHOD OF APPLICATION- On the day of its use, minimal top agar, containing 0.8 % agar (W/V) and 0.5 % NaCl (W/V), was melted and supplemented with L-histidine, D-biotin and L-tryptophan solution to a final concentration of 50 µM each. Top agar not used with S9 or Sham mix was supplemented with 25 mL of water for each 100 mL of minimal top agar. For the preparation of media and reagents, all references to water imply sterile, deionized water produced by the Milli-Q Reagent Water System. Bottom agar was Vogel-Bonner minimal medium E (Vogel and Bonner, 1956) containing 1.5% (W/V) agar. Nutrient bottom agar was Vogel-Bonner minimal medium E containing 1.5% (W/V) agar and supplemented with 2.5% (W/V) Oxoid Nutrient Broth No. 2 (dry powder). Nutrient Broth was Vogel-Bonner salt solution supplemented with 2.5% (W/V) Oxoid Nutrient Broth No. 2 (dry powder). Each plate was labeled with a code system that identified the test article, test phase, dose level, tester strain, and activation, as described in detail in BioReliance's Standard Operating.- Procedures. Test article dilutions were prepared immediately before use. One-half (0.5) mL of S9 or Sham mix, 100 µL of tester strain and 50 µL of vehicle or test article dilution were added to 2.0 mL of molten selective top agar at 45±2°C. After vortexing, the mixture was overlaid onto the surface of 25 mL of minimal bottom agar. When plating the positive controls, the test article aliquot was replaced by a 50 µL aliquot of appropriate positive control. After the overlay had solidified, the plates were inverted and incubated for approximately 48 to 72hours at 37±2°C. Plates that were not counted immediately following the incubation period were stored at 2-8°C until colony counting could be conducted. The condition of the bacterial background lawn was evaluated for evidence of test article toxicity by using a dissecting microscope. Precipitate was evaluated by visual examination without magnification. Revertant colonies for a given tester strain and activation condition, except for positive controls, were counted either entirely by automated colony counter or entirely by hand unless the assay was the preliminary toxicity assay or the plate exhibited toxicity. Plates with sufficient test article precipitate to interfere with automated colony counting were counted manually.
- Evaluation criteria:
- For the test article to be evaluated positive, it must cause a dose-related increase in the mean revertants per plate of at least one tester strain over a minimum of two increasing concentrations of test article. Data sets for tester strains TA1535 and TA1537 were judged positive if the increase in mean revertants at the peak of the dose response is equal to or greater than three times the mean vehicle control value. Data sets for tester strains TA98, TA100 and WP2 uvrA were judged positive if the increase in mean revertants at the peak of the dose response is equal to or greater than two times the mean vehicle control value.All criteria for a valid test were met. To meet this criteria, all Salmonella tester strain cultures must demonstrate the presence of the deep rough mutation (rfa) and the deletion in the uvrB gene. Cultures of tester strains TA98 and TA100 must demonstrate the presence of the pKM101 plasmid R-factor. All WP2 uvrA cultures must demonstrate the deletion in the uvrA gene. All cultures must demonstrate the characteristic mean number of spontaneous revertants in the vehicle controls as follows (inclusive): TA98, 10-50; TA100, 80-240; TA1535, 5-45; TA1537, 3-21; WP2uvrA, 10-60. To ensure that appropriate numbers of bacteria are plated, tester strain culture titers must be greater than or equal to 0.3E09 cells/mL. The mean of each positive control must exhibit at least a three-fold increase in the number of revertants over the mean value of the respective vehicle control. A minimum of three non-toxic dose levels are required to evaluate assay data. A dose level is considered toxic if one or both of the following criteria are met: (1) A >50 % reduction in the mean number of revertants per plate as compared to the mean vehicle control value. This reduction must be accompanied by an abrupt dose-dependent drop in the revertant count. (2) A reduction in the background lawn.
- Statistics:
- For each replicate plating, the mean and standard deviation of the number of revertants per plate were calculated and are reported.
Results and discussion
Test results
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: With metabolic activation: > 5000 ug/plate Without metabolic activation: > 5000 ug/plate
- Remarks on result:
- other: other: Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, and Escherichia coli WP2 uvrA
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
For a summary of results: See Table 5.5.2 (Summary of Results) in document attached to Chapter 1.11 Additional Remarks.
Dose (mg/plate) |
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvrA |
Liver Microsomes: None |
|||||
Vehicle |
17±3 |
96±11 |
13±2 |
5±3 |
17±3 |
75 |
16±3 |
99±10 |
9±4 |
5±0 |
15±3 |
200 |
18±2 |
105±16 |
9±3 |
5±3 |
14±2 |
600 |
15±3 |
108±15 |
11±1 |
5±1 |
15±2 |
1800 |
15±3 |
89±18 |
14±4 |
6±1 |
11±2 |
5000 |
11±3 |
95±13 |
11±2 |
6±1 |
13±2 |
Positive |
91±13 |
332±10 |
195±7 |
448±50 |
77±10 |
Dose (mg/plate) |
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvrA |
Liver Microsomes: Rat Liver S9 |
|||||
Vehicle |
18±4 |
90±4 |
8±4 |
7±1 |
11±2 |
75 |
16±6 |
88±10 |
10±1 |
6±3 |
11±2 |
200 |
16±5 |
97±7 |
10±3 |
6±1 |
14±2 |
600 |
20±1 |
92±11 |
10±3 |
5±3 |
11±1 |
1800 |
15±3 |
85±7 |
11±2 |
5±3 |
12±1 |
5000 |
15±3 |
84±9 |
10±2 |
4±2 |
10±1 |
Positive |
279±134 |
356±19 |
50±8 |
32±11 |
86±8 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):negativeUsed for data submission
- Executive summary:
Used for data submission
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