Aluminum magnesium vanadium oxide

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 - 17 Apr 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Chinese National Environmental Protection Agency, the Guidelines for the Testing of Chemicals, 2004, 207 Earthworm, Acute Toxicity Test

Version / remarks:

2004

GLP compliance:

no

Remarks:

according to Chinese GLP (GB/T 22275.1-2008); comparable to OECD GLP C(97)186/FINAL

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): Since the test substance is insoluble in water and suitable solvents, it was directly applied and mixed in silicia sand after weighting with a balance. The treated sand was mixed thoroughly with artificial soil and then was moistured with deionized water to obtain an equal distribution of the test substance in the test substrate

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Shanghai Yinquan Agriculture Development Co., Ltd
- Age at test initiation (mean and range, SD): adult (with citellum)
- Weight at test initiation (mean and range, SD): 300 - 600 mg
- The newly acquired earthworms were quarantined and observed for 14 day prior to testing
- Earthworms were bred in cow dung and kept in uncontaminated artificial soil under 20 °C for 7 days prior testing

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

Initial: 20.2 °C
End: 20.7 °C

pH:

Initial: 6.15
End: 6.17

Moisture:

Initial: 30.87% dry soil weight
End: 29.57% dry soil weight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 L glass beakers covered with a film to avoid drying-out
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 600 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The number of dead and affected worms was observed at 7 and 14 day after test start.

TEST CONCENTRATIONS
- Range finding study:
- Test concentrations: 10, 100 and 1000 mg/kg soil dw
- Results used to determine the conditions for the definitive study: no mortality at any of the test concentrations

Nominal and measured concentrations:

control and 1000 mg/kg dw soil (nominal)

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: no mortality occurred
- Total mass of adults at beginning of test: control: 1670 mg; 1000 mg/kg: 1590 mg
- Total mass of adults at end of test: control: 1610 mg; 1000 mg/kg: 1570 mg

Results with reference substance (positive control):

LC50 (14d) = 10.82 mg/kg dw (CI: 9.75 - 11.99 mg/kg dw)
Test performed 6 - 20 Sep 2012

Reference 2

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

non GLP study

Qualifier:

according to guideline

Guideline:

other: ISO guideline 10872:2020

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): 4 g of dry soil were thoroughly mixed in 10-ml glas jars with the respective mass of test item
- Controls: Lufa St. 2.2 soil without test item

Test organisms (species):

Caenorhabditis elegans

Animal group:

nematods

Details on test organisms:

TEST ORGANISM
- Common name: nematode
- Source: Caenorhabditis Genetic Centre, University of Minnesota
- Age at test initiation: J1 juvenile stages

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

96 h

Test temperature:

19.5 – 20.5 °C;

pH:

5.4 (± 0.1)

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 24-well multidishes
- Amount of soil or substrate: 4 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8 (soil test 1), 6 (soil test 2)


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil classification system: loamy sand
- Composition (if artificial substrate): Particle size distribution: <0.002 mm: 6.4 (± 0.9) %; 0.002-0.05 mm: 12.2 (± 0.6) %; 0.05-2.0 mm: 81.4 (± 1.2) %
- Organic carbon (%): 2.16 % (± 0.40)
- Maximum water holding capacity: 44.8 (± 5.0) g/100 g

OTHER TEST CONDITIONS
- Photoperiod: in the dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth adn reproduction after 96 hours of exposure

Nominal and measured concentrations:

640, 1600, 4000, 10000, 25000, 50000 mg/kg dry material (nominal)

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 25 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

4 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

96 h

Dose descriptor:

EC10

Effect conc.:

1 824 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

16 582 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

- No. of offspring produced: ≥192 (control), ≥ 34 (treatment)

Reported statistics and error estimates:

The data analysis was performed with one-way ANOVA (post-hoc test: Dunnett, Games-Howell), descriptive statistics, dose-response curves fitted to %inhibition data of growth and reproduction.

Description of key information

LC50 (14d) > 1000 mg/kg soil dw (OECD 207, Eisenia fetida)

EC10 (96h) = 1824 mg/kg soil dw (ISO 10872:2020, Caenorhabditis elegans)

Additional information

One study is available on the short-term toxicity of Aluminum magnesium vanadium oxide to soil macroorganisms (2013). This study was conducted as a limit test according to OECD Guideline 207 and Chinese GLP. The test organism Eisenia fetida was exposed to the single test concentration of 1000 mg/kg dw in artificial soil. No mortality occurred and the LC50 was > 1000 mg/kg soil dw.

One supporting study is available on the long-term toxicity of Aluminum magnesium vanadium oxide to soil macroorganisms (2020). This study was not conducted under GLP conditions according to ISO Guideline 10872:2020. The test organism Caenorhabditis elegans was exposed to the test concentrations of 640, 1600, 4000, 10000, 25000, 50000 mg/kg dw in artificial soil. The NOEC based on growth and reproduction were determined to be≥25000 and 4000 mg/kg dws, respectively. The EC10 based on growth was determined to be 1824 mg/kg dws.