Aluminum magnesium vanadium oxide

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 - 19 Mar 2013

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

other: SEPA, The Guildelines for the Testing of Chemicals, 2004, 203 Fish Acute Toxicity Test

GLP compliance:

no

Remarks:

according to Chinese GLP (GB/T 22275.1-2008); comparable to OECD GLP C(97)186/FINAL

Analytical monitoring:

yes

Details on sampling:

- Concentrations: blank and 100 mg/L
- Sample storage conditions before analysis: samples were analysed directly

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is a metal compound which contains Al, V and Mg. A loading rate of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark at 700 rpm for 72 h. The test solution was then filtered by using a 0.45 μm nitrocellulose membrane

Test organisms (species):

other: Gobiocypris rarus

Details on test organisms:

TEST ORGANISM
- Common name: Chinese rare minnow
- Source: Research Center for Eco-Environmental Science, Chinese Academy of Sciences (ID: GR-IHB-120919)
- Length at study initiation (length definition, mean, range and SD): mean: 1.7 cm; SD: 0.19 cm
- Weight at study initiation (mean and range, SD): mean: 0.13 g; SD: 0.02 g
- Feeding during test:
Food type: Brine Shrimp Aquarium Flake Food, O.S.I®
Amount: approximately 1% of body weight per day
Frequency: twice a day

ACCLIMATION
- Acclimation period: > 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Brine Shrimp Aquarium Flake Food, O.S.I®
- Feeding frequency: daily
- Health during acclimation (any mortality observed): No diseases were observed

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

14 d

Hardness:

165 mg CaCO3/L

Test temperature:

21.1 - 22.9 °C

pH:

7.55 – 8.93

Dissolved oxygen:

62.9% - 92.1% ASV

Nominal and measured concentrations:

Nominal concentration: control, 100 mg/L (loading rate)
Measured concentrations: see Any other information on results incl. tables

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 5 L glass tanks
- Aeration: continuously
- Renewal rate of test solution (frequency/flow rate): 48 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.16 g fish/litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water dechlorinated by activated carbon and sterilized by UV lamp
- Intervals of water quality measurement: pH, dissolved oxygen and temperature: at 2 days intervals

OTHER TEST CONDITIONS
- Photoperiod: Light : Dark = 12 : 12 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortalities and sublethal effects were observed and recorded daily

Reference substance (positive control):

no

Duration:

14 d

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

loading rate

Basis for effect:

mortality

Key result

Duration:

14 d

Dose descriptor:

NOELR

Effect conc.:

< 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Remarks:

loading rate

Basis for effect:

growth rate

Details on results:

- Observed effects at each concentration for each observation time: No mortality occurred during the study, but the treatment had a significant effect on the specific growth rate of the fish (see Any other information on results incl. tables).

Reported statistics and error estimates:

The data analysis was performed with software TOXCALC (v5.0.26).

Measured concentrations of Al in the test solutions

Group		0d	2d - aged	2d - new	4d - aged	4d - new	6d - aged	6d - new	Mean (mg/L)
Control [mg/L]	1	0.0148	0.00418	0.00575	0.0135	0.0124	0.00829	0.0150	-	-
	2	0.0141	0.00487	0.00591	0.0122	0.0109	0.00639	0.0151	-
	3	0.0147	0.00537	0.00613	0.0109	0.00983	0.00565	0.0153	-
100 mg/L loading rate	1	4.40	1.63	1.70	1.69	1.24	1.03	1.70	-	-
	2	4.44	1.56	1.76	1.98	1.19	1.09	1.88	-
	3	4.44	1.56	1.74	1.89	1.28	0.984	1.82	-
Group		8d - aged	8d - new	10d - aged	10d - new	12d - aged	12d - new	14d - aged	Mean (mg/L)
Control group [mg/L]	1	0.0150	0.00998	0.00969	0.0140	0.0126	0.0131	0.0128	-	-
	2	0.0129	0.0136	0.00961	0.0133	0.0130	0.0133	0.0200	-
	3	0.0124	0.0133	0.00904	0.0127	0.0120	0.0133	0.0170	-
100 mg/L loading rate	1	1.40	1.67	1.61	1.78	1.56	1.57	1.47	1.71	1.74
	2	1.37	1.66	1.62	1.85	1.55	1.54	1.48	1.75
	3	1.54	1.67	1.71	1.80	1.54	1.56	1.48	1.75

“-”, not available.

Measured concentrations of V in the test solutions

Group		0d	2d - aged	2d - new	4d - aged	4d - new	6d - aged	6d - new	Mean (mg/L)
Control group [mg/L]	1	0.000199	0.000587	0.000622	0.000380	0.000315	0.000288	0.000501	-	-
	2	0.000174	0.000552	0.000623	0.000439	0.000308	0.000467	0.000474	-
	3	0.000178	0.000643	0.000663	0.000267	0.000321	0.000413	0.000476	-
100 mg/L loading rate	1	1.40	0.564	0.584	0.584	0.480	0.468	0.568	-	-
	2	1.42	0.584	0.584	0.584	0.480	0.488	0.568	-
	3	1.44	0.584	0.584	0.584	0.480	0.472	0.568	-
Group		8d - aged	8d - new	10d - aged	10d - new	12d - aged	12d - new	14d - aged	Mean (mg/L)
Control group [mg/L]	1	0.000507	0.000448	0.000352	0.000383	0.000414	0.000283	0.000240	-	-
	2	0.000388	0.000908	0.000234	0.000315	0.000387	0.000332	0.000331	-
	3	0.000526	0.000414	0.000355	0.000368	0.000342	0.000364	0.000472	-
100 mg/L loading rate]	1	0.540	0.524	0.516	0.508	0.488	0.500	0.504	0.578	0.588
	2	0.540	0.520	0.512	0.524	0.496	0.500	0.500	0.601
	3	0.544	0.528	0.512	0.520	0.488	0.500	0.508	0.585

“-”, not available.

Measured concentrations of Mg in the test solutions

Group		0d	2d - aged	2d - new	4d - aged	4d - new	6d - aged	6d - new	Mean  (mg/L)
Control group [mg/L]	1	8.49	7.05	7.73	8.26	7.24	8.36	7.73	-	-
	2	8.75	8.38	7.78	7.89	7.64	8.18	7.69	-
	3	8.55	8.76	7.58	7.95	7.53	8.24	7.66	-
100 mg/L loading rate	1	15.8	14.9	15.4	16.4	14.2	16.0	15.9	-	-
	2	15.7	14.6	16.6	16	14.4	16.0	16.1	-
	3	15.9	16.3	16.1	16.7	14.3	15.7	16.1	-
Group		8d - aged	8d - new	10d - aged	10d - new	12d - aged	12d - new	14d - aged	Mean  (mg/L)
Control group [mg/L]	1	7.10	7.08	7.79	7.36	7.59	7.19	7.47	-	-
	2	6.92	7.08	8.21	7.25	7.45	7.11	7.34	-
	3	7.10	7.06	8.11	7.28	7.75	7.33	7.30	-
100 mg/L loading rate	1	14.8	14.7	14.9	15.0	15.1	15.2	15.3	15.3	15.3
	2	14.8	14.7	14.4	14.9	14.9	14.9	15.0	15.3
	3	14.6	14.6	14.9	15.0	14.9	15.0	15.2	15.4

“-”, not available.

The body weight and tank-average specific growth rate of test fish in the control group after 0 d and 14 d

Blank control Replicates	Body weight (g) after 0d						Body weight (g) after 14d						Tank-average specific growth rate (%)
	1	2	3	4	5	Mean	1	2	3	4	5	Mean
1	0.1454	0.1275	0.1084	0.0981	0.1544	0.1267	0.1480	0.1562	0.1296	0.1477	0.1446	0.1452	0.9733
2	0.1359	0.1204	0.1515	0.1461	0.1505	0.1409	0.1452	0.1522	0.1487	0.1403	0.1494	0.1472	0.3114
3	0.1181	0.0999	0.1343	0.1053	0.1553	0.1226	0.1214	0.1914	0.1416	0.1586	0.0744	0.1375	0.8201

 

The body weight and tank-average specific growth rate of test fish in treatment groups after 0 d and 14 d

Treatment group		Body weight (g) after 0d						Body weight (g) after 14d						Tank-average specific growth rate (%)
		1	2	3	4	5	Mean	1	2	3	4	5	Mean
100.0 mg/L	1	0.1553	0.1558	0.1189	0.1164	0.1049	0.1303	0.0922	0.0792	0.0997	0.1103	0.1116	0.0986	-1.5774
	2	0.1201	0.1068	0.0946	0.1336	0.1098	0.1130	0.1074	0.1187	0.1175	0.0696	0.0778	0.0982	-1.0018
	3	0.1437	0.1040	0.1448	0.1171	0.1439	0.1307	0.1015	0.0873	0.0589	0.1038	0.0986	0.0900	-1.5875

Description of key information

NOELR(14d, growth rate) < 100 mg/L (nominal, loading rate) (OECD 204, Gobiocypris rarus). Measured concentration of Al, V, and Mg were 1.74 mg/L, 0.588 mg/L, and 15.3 mg/L

Additional information

One study is available on the long-term toxicity of Aluminium magnesium vanadium oxide to fish. This key study was conducted as a semi-static limit test according to OECD Guideline 204 and Chinese GLP (2013). The test organism Gobiocypris rarus was exposed to a filtered test item solution of nominal 100 mg/L (loading rate) for 14 days. No mortality occurred during the study, but a significant difference in specific growth rate was observed between the control and the treatment group. The average recoveries of Al spiked 0.0200 mg/L and 0.160 mg/L samples were 117% and 103% with RSDs of 7.32% and 2.35%, respectively. The average recoveries of V spiked 0.0200 mg/L and 0.160 mg/L sample were 101% with RSDs of 1.24% and 1.13%, respectively. The average recoveries of Mg spiked 15 mg/L and 30 mg/L sample were 84.9% and 83.6% with RSDs of 1.04% and 0.77%, respectively. The NOELR based on mortality was determined to be 100 mg/L. For growth inhibition, the NOELR is below 100 mg/L. This corresponds to geometrich mean measured concentrations of Al, V and Mg of 1.74, 0.588, and 15.3 mg/L, respectively