3-chloroaniline

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 84/449/EWG

Principles of method if other than guideline:

Five male and 5 female Wistar rats were occlusively applied 1000 or 2000 mg m-chloroaniline/kg bw. The test substance was applied on gauze covering about 10% of the body surface and caped with aluminium foil. After 24 hours the test substance was remouved with lukewarm water. The animals were observed for mortality, clinical signs and body weight gain during a post-observation period of 14 days. a pathological examination was performed on all animals which died during the observation period or were sacrificed at the end of the study period.

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

3-chloroaniline

EC Number:

203-581-0

EC Name:

3-chloroaniline

Cas Number:

108-42-9

Molecular formula:

C6H6ClN

IUPAC Name:

3-chloroaniline

Test material form:

other: liquid

Details on test material:

CAS no.: 108-42-9
purity: 99.5%
molecular weight. 127.6 g/mol
appearance: weak yellow liquid
density: 1.21 g/m³

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

polyethylene glycol

Duration of exposure:

24 hours

Doses:

1000 or 2000 mg/kg bw

No. of animals per sex per dose:

5 male and 5 female animals/dose

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

> 1 000 - < 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 2 000 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

Signs of intoxicition at a dose of 2000 mg/kg bw were cyanosis and sedation on male and female rats. A single female rats showed at a dose of 1000 mg/kg bw cyanosis, gaunt flanks and a bad general condition. All other animals revealed no symptoms at a dose of 1000 mg/kg bw.

Pathological signs of the deceased animals at doses of 2000 mg/kg bw were changes of the gastrointestinal tract, brownish discoloured lungs and the urinary bladder was filled plump with liquid on males and one female. The gastrointestinal tract was partly black discoloured on 2 females and one female showed a clay-coloured liver. The animals which died during the post-observation period were cyanotic. All animals sacrificed at the end of the study revealed no pathological signs.

The LD50 is > 1000 and < 2000 mg/kg bw for female rats and ca. 2000 mg/kg bw for male rats.

Applicant's summary and conclusion

Executive summary:

Five male and 5 female Wistar rats were occlusively applied 1000 or 2000 mg m-chloroaniline/kg bw. The test substance was applied on gauze covering about 10% of the body surface and caped with aluminium foil. After 24 hours the test substance was remouved with lukewarm water. The animals were observed for mortality, clinical signs and body weight gain during a post-observation period of 14 days. A pathological examination was performed on all animals which died during the observation period or were sacrificed at the end of the study period.

Signs of intoxicition at a dose of 2000 mg/kg bw were cyanosis and sedation on male and female rats. A single female rats showed at a dose of 1000 mg/kg bw cyanosis, gaunt flanks and a bad general condition. All other animals revealed no symptoms at a dose of 1000 mg/kg bw.

The LD50 is > 1000 and < 2000 mg/kg bw for female rats and ca. 2000 mg/kg bw for male rats.