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EC number: 617-116-8 | CAS number: 80573-04-2
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 June - 16 July 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Test material: Balsalazide acid
- Appearance: Slightly yellow brown powder
- Batch number: 0400
- Expiry date: April 2019
- Purity: >98%
- Storage: Room temperature
- Stability: 12 months - Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from the treated vessels at the start and end of the test.
- Vehicle:
- no
- Details on test solutions:
- The test solution was sonicated for 40 minutes to homogenise the sample.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Breeder in Eurofins Biolab Test Facility
- Age: 6 - 24 hours
- Feeding during test: None
ACCLIMATION
- Type and amount of food: Green alga Pseudokirchneriella subcapitata and Saccharomices cerevisiae
- Water change: 3 times per week
- Photoperiod: 16 hours of light, 8 hours of darkness - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- Between 140 and 250 mg CaCO3/L
- Test temperature:
- 20°C ± 2°C
- pH:
- Control: 7.83 - 7.93
Test concentration: 7.10 - 7.67 - Dissolved oxygen:
- > 3 mg/L
- Nominal and measured concentrations:
- Range finding test: Ten fold dilutions from 100 mg/L to 0.01 mg/L
Definitive test: 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker (500 mL)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Aeration: None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water containing:
- Bihydrated calcium chloride: 11.76 g/L
- Eptahydrated magnesium sulphate: 4.93 g/L
- Acid sodium bicarbonate: 2.59 g/L
- Potassium chloride: 0.23 g/L
- Deionised water: Up to 1000 mL volume
OTHER TEST CONDITIONS
- Photoperiod: None
RANGE-FINDING STUDY
- Test concentrations: Ten-fold dilutions from 100 mg/L to 0.01 mg/L
- Results used to determine the conditions for the definitive study: Yes
EFFECT PARAMETERS MEASURED: The immobilisation of the test organisms was evaluated at 24 and 48 hours.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Stability of the test item: The sample is considered stable because the measured concentrations of the test item at the end of the test have been maintained within ± 20% at the beginning of the test (see Table 1).
- Validity criteria fulfilled:
- yes
- Remarks:
- Immobilised Daphnia in the control: 0%
- Conclusions:
- According to the results of the test, the EC50 (48 hours) for invertebrates is estimated to be >100 mg/L based on nominal concentrations of the test item “Balsalazide acid”.
Reference
Table 1: Stability of the test item
Nominal concentration |
Measured concentration (mg/L) |
% concentration Variation |
Stability |
|
T=0 |
T=48 h |
|||
Treated (100 mg/L) |
95.5 |
93.4 |
2.11 |
Stable |
Description of key information
A GLP study was performed in accordance with OECD Guideline 202 to assess the toxicity of the test material, Balsalazide acid, to Daphnia magna. According to the results of the test, the EC50 (48 h) was > 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
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