Registration Dossier
Registration Dossier
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EC number: 201-163-2 | CAS number: 78-97-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (GLP), detailed documentation not available
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1993
Materials and methods
- Principles of method if other than guideline:
- Method: other: Japanese Guideline for Screening Mutagenicity Testing of Chemicals
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Lactonitrile
- EC Number:
- 201-163-2
- EC Name:
- Lactonitrile
- Cas Number:
- 78-97-7
- Molecular formula:
- C3H5NO
- IUPAC Name:
- 2-hydroxypropanenitrile
- Details on test material:
- 2-Hydroxypropanenitrile, commercial
Purity: 92.3 %
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- S. typhimurium: 4.69, 9.38, 18.75, 37.5, 75, 150 ug/plate, E. coli WP2 uvrA: 75, 150, 300, 600, 1200 or 2400 ug/plate
- Details on test system and experimental conditions:
- IUCLID4 Type: Ames test
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks on result:
- other: other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538, E. coli WP2 uvrA
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Cytotoxicity
With metabolic activation: 150 pg/plate (TA), 1500 ug/plate (WP2)
Without metabolic activation: 150 ug/plate (TA), 2400 ug/plate (WP2)
Precipitation
negative
Mutagenicity
With metabolic activation: negative for all strains
Without metabolic activation: negative for all strains
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
