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EC number: 939-060-6
CAS number: -
The mutagenic potential of Initiator 94 is determined with the Bacterial
Reverse Mutation Test according to OECD 471/ EU-guideline B.13/14. Two
valid experiments were performed, a first one using the plate
incorporation method and a second one with the pre-incubation method. In
both experiments five strains of Salmonella typhimurium: TA 97a, TA 98,
TA 100, TA 102 and TA 1535, were used with and without metabolic
activation by S9. Per strain and dose 4 plates with and 4 plates without
metabolic activation were used.
In the first experiment five concentrations of the test item, ranging
from 50 to 5015 µg/plate, were used and incubated for 48 hours.
In the second experiment five concentrations of the test item, ranging
from 313 to 5001 µg/plate, were used and incubated for 48 hours.
In both experiments no signs of toxicity towards the bacteria and no
mutagenic effects could be observed. The sterility control, the negative
controls and the positive controls were within the range of historical
Therefore, under the conditions of this test, the test item is
considered not mutagenic.
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