[No public or meaningful name is available]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22.08.2012 - 05.11.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well documented GLP study according to Guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

not applicable for UVCB substances

EC Number:

939-060-6

IUPAC Name:

not applicable for UVCB substances

Test material form:

other: liquid

Details on test material:

- Name of test material: Initiator 94 RX076594
- Batch: 004IN11
- Production date: 16.05.2011
according to certificate of analysis and its addendum of 1. August 2012:
purity: 32% (main component and active species: benzpinakolsilylethers)
further composition: Phosphoric acid tributylester (Tributylphosphat) 19.3%, 1,2-Benzenedicarboxylic acid-di-2-propenylester (Diallylphthalate) 8.7%; Methylbenzene (toluol) 12.9%; Diphenylmethanone (Benzophenon) 12.4 %; 1,1,2,2-Tetraphenylethane-1,2-diol (Benzpinakol) 14.6%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: sewage effluent from muinicipal wastewater treatment plant Borsdorf locality Panitzsch.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral Medium according to OECD 301 F
- Test temperature: 21.8-22.5 °C
- pH: 7.52 - 9.34
- pH adjusted: no


TEST SYSTEM
- Culturing apparatus: 500 mL test flasks made from brown glass
- Number of culture flasks/concentration: 2 flasks containing test item (100 mg/L) , mineral medium and inoculum
- Measuring equipment: - pH-meter, automatic Respirometer with measuring Oxygen uptake hourly, Temperature control, Total organic Carbon analyzer
- Details of trap for CO2: Potassium hydroxide pellets


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (mineral medium + inoculum)
- Reference: yes (Reference item + mineral medium + inoculum)
- Toxicity control: test item and reference item (Sodium acetate anhydrous) + mineral medium + inoculum

Other:
Nitrification is not relevant, as the test item does not contain nitrogen.
ThOD: could not be calculated due to the unknown formula of the test items main constituents. Instead Chemical Oxygen Demand (COD) was determined with potassium dichromate and concentrated sulfuric acid according to DIN 38409-41:1980-12.
Preparation of test item: homogenized by heating to 40°C and shaked

Reference substance

Reference substance:

other: Sodium acetate anhydrous

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

15.2

Sampling time:

28 d

Remarks on result:

other: based on COD

BOD5 / COD results

BOD5 / COD

Parameter:

COD

Value:

2.02 g O2/g test mat.

Results with reference substance:

68.8 % degradation within 28 days.
pass level was reached in 2 days.
10 day window was met (60 % biodegradation)

Any other information on results incl. tables

Toxicity control, containing both the test item and the reference item showed 32 % degradation within 14 days, which is more than 25%.

Oxygen uptake of the inoculum blank was 1.6 mg/L in 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Executive summary:

To determine the ready biodegradability, the test item was tested according to OECD 301 F Manometric Respirometry Test. Test item concentration: 100 mg/L, reference item: 100 mg/L soldium acetate, toxicity control: both test item and reference item and inoculum (from sewage effluent) blank were proved each in duplicate. The maximum removal of test item was 15.2% based on COD. Therefore, the test item is regarded as not readily biodegradable.

All validity criteria were met. The study is considered reliable without restriction.