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The study was performed to assess the acute dermal toxicity of E Stage 3 intermediate in Sprague Dawley rats. The mehtod was designed to meet the guidelines for the testing of chemicals "Acute Dermal Toxicity" (TG 402) published by the Ministry of Environmental Protection of People's Republic of China in the year of 2013.
A limit test at one dose level of 2000mh/kg body weight was carried out via dermal route in a group of 10 animals (5 males and 5 females). Clinical observations were made during the first 30 minutes and a 1, 2, and 4 hours after dosing and then once each day for up to 14 days. Individual weights of animals were determined within 24 hours of arrival, at grouping, on Day 0 (day of dosing), Day 7 and Day 14. At the end of the test, a gross necropsy was performed on all animals under test.
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