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EC number: -
CAS number: -
A fish acute toxicity study of E stage 3 intermediate was conducted
with Zebra fish (Danio rerio) over a 96 h exposure period.
Based on the results of the preliminary test, the definitive test was
carried out at a saturated concentration of 100 mg/L, 50% saturated
concentration, 25% saturated concentration, 12.5% saturated
concentration and 6.25% saturated concentration. The actual
concentration of test solutions was measured by HPLC/MS/MS method. A
concurrent blank control was performed in parallel. 7 fish were used for
the test and control group.
Based on the results of the preliminary test, a semi-static, renewed at
48 h with solution was used in the definitive test. Dissolved oxygen, pH
and temperature were measured daily during the 96 h exposure period in
the control and test solution. Temperature (in the water bath) was
measured continuously by an automatic monitoring sytem.
During the 96 h exposure period no death or visible toxicity signs were
observed in the control group and test group of 6.25% saturated
concentration. 2 fish died in test group 12.5% saturated concentration
and 7 fish died in other test groups.
The concentrations of the test system were determined in samples of test
media by HPLC/MS/MS method. The measured concentrations were as follows:
The mean concentrations in fresh media were 1.66, 3.09, 6.37, 13.1 and
The mean concentrations in old media were 1.38, 2.71, 5.63, 11.1 and
The overall mean concentrations were 1.52, 2.90, 6.00, 12.1 and 27.8mg/L.
Based on the results, the 96 hour LC50 of E stage 3
intermediate to Zebra fish was 3.43 mg/L, 95% confidence limits 2.7 -
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