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EC number: 439-210-6
CAS number: 554-62-1
Invertebrates: 48h-EC50 (Daphnia magna, immobility) = 131 µg/L
Algae: 72h-ErC50 (Pseudokirchneriella subcapitata, growth rate) =
166 µg/L; 72h-ErC10 (Pseudokirchneriella subcapitata, growth rate) =
45.5 µg/L (mean measured)
Acute aquatic toxicity
Reliable key studies are available for aquatic algae (according to OECD
201, GLP) and invertebrates (according to OECD 202, GLP). Adverse
effects of the submission substance were observed in both species, algae
and invertebrates. The validity criteria of the guidelines are fulfilled
and results considered reliable and adequate for the aquatic hazard
Inhibition of growth (r) and inhibition of yield (b) of aquatic algae
were observed in a dose-response relationship during the 72 hours
exposure period. No adverse effects were observed in the two lowest test
concentration, i.e. 8.27 and 12.7 µg/L. Derived effect concentrations on
growth inhibition were: 72h-ErC50 = 166 µg/L, 72h-ErC10 = 45.5 µg/L, and
72h-NOErC = 12.7 µg/L (based on geom. measured concentrations). The
derived effect concentrations on yield of biomass were: 72h-EbC50 = 50.1
µg/L, 72h-EbC10 = 18.8 µg/L, and 72h-NOEbC = 12.7 µg/L (based on geom.
measured concentrations). Microscopic evaluation of the cells at the
start and end of exposure revealed no morphological abnormalities.
Immobilisation of Daphnia magna was observed in a dose-response
relationship during the 48 hours exposure period. The derived effect
concentrations were: 48h-EC50 = 0.131 mg/L (95 % confidence limits:
0.106 - 0.161 mg/L).
In conclusion, the most sensitive species concerning short-term toxicity
is Daphnia magna.
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