(3-chloroprop-1-enyl)benzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985-10-01 to 1985-10-25

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Comparable to OECD Guideline 404 study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

Principles of method if other than guideline:

This study follows the guidelines published in the Federal Register Vol. 43 No. 163 Part IV, EPA July 26, 1979.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(3-chloroprop-1-enyl)benzene

EC Number:

220-246-4

EC Name:

(3-chloroprop-1-enyl)benzene

Cas Number:

2687-12-9

Molecular formula:

C9H9Cl

IUPAC Name:

(3-chloroprop-1-enyl)benzene

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study reports): JNJ-118872-AAA (T000749)
- Physical state: liquid
- Appearance: Light yellow, light brown liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Remarks:

Albino

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Laboratory in-house stock colony
- Age at study initiation: No data
- Weight at study initiation: 4.35 - 4.45 kg
- Housing: Housed in a wire cage in compliance with AALAC regulations.
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: No data


ENVIRONMENTAL CONDITIONS: no data


IN-LIFE DATES: No data

Test system

Type of coverage:

open

Preparation of test site:

other: intact or abraded

Vehicle:

not specified

Controls:

other: the back of the rabbits with intact skin

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): undiluted

VEHICLE: no data

Duration of treatment / exposure:

Single dose for 6 hour exposure

Observation period:

after 24 hours, 2, 3, 4, 5, 6, 7, 10 and 14 days, etc. until reactions proved to be reversible.

Number of animals:

Two males

Details on study design:

TEST SITE
- Area of exposure: The skin at the right side of the back was abraded, whereas the left side was left intact. A definite sample was applied for each side to an area of 1" x 1" skin square on the intact and on the abraded skin.
- % coverage: No data
- Type of wrap if used: Not applicable.


REMOVAL OF TEST SUBSTANCE
- Washing (if done): The materials were flushed and washed with handwarm water and gently dried.
- Time after start of exposure: after 6 hours


SCORING SYSTEM:
The reactions were evaluated in both the group with intact skin and the group with abraded skin after 24 hours, 2, 3, 4, 5, 6, 7, 10 and 14 days, etc. until reactions proved to be reversible. The Primary Irritation Index (PII) and Classification was according to Draize and evaluated after 24 and 72 hours of application.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.67

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: Intact skin

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.33

Max. score:

4

Reversibility:

not fully reversible within: 13 days (score 1 on days 7, 10 and 13)

Remarks on result:

other: Intact skin

Results 3

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: Intact skin

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.33

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: Intact skin

Results 5

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.67

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: abraded skin

Results 6

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.33

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: abraded skiin

Results 7

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: abraded skin

Results 8

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.33

Max. score:

4

Reversibility:

not fully reversible within: 13 days

Remarks on result:

other: abraded skin

Irritant / corrosive response data:

T 749 resulted in a slight irritation to the skin of the rabbits.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

Under the conditions of this study, the test item resulted in a slight irritation to the skin of the rabbits. Based on the results of this study and the criteria of the CLP Regulation, the substance is classified as irritating to the skin based on persisting inflammation observed until day 14 which is reflected in the scoring of erythema and edema until day 14.