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EC number: 947-377-6
CAS number: -
4.10.1: Results obtained on replicate one (after shaking 72h to saturate
the solution used)
Mean ± SD (mN/m)
72.67 ± 0.07
Test substance: First series
66.39 ± 0.16
Test substance: Second series (verification)
67.71 ± 0.17
4.10.2: Results obtained on replicate two (after shaking 7 days to
saturate the solution used)
72.64 ± 0.05
68.28 ± 0.26
68.17 ± 0.18
surface tension of the negative control was determined with 72.67 mN/m
at 20.4°C and 72.64 mN/m at 20.6°C, therefore showing a difference of
0.01% from the stated literature value, calculated from the
equation cited in the « online calculation of the Surface Tension of
Ordinary Water Substance » page
72.68 mN/m at 20.4°C and 72.64 mN/m at 20.3°C. The difference lies
within the accepted range of 1% deviation from literature value.
both replicates, detailed findings of each step are provided in the
study report. Values ranging from 66.28 mN/m to 68.11 mN/m (replicate
one) and from 67.89 mN/m to 68.84 mN/m (replicate two) were recorded
showing no significant variations.
The values of the single determinations show no significant difference,
i.e. the difference between the mean values obtained in both measurement
series was 1.99 % for replicate one and 0.16% for replicate two. Also
the difference between the mean values obtained with the two replicates
was 2.85% laying within the criterion validity of 5%.
dependence on time was observed.
of TOC analysis of the filtrated test solutions 90%:
analysis of the filtrated test solutions was performed using the carbon
analyser TOC after monthly calibration and quality control after every
4.10.3 Estimation of the concentration of the test item in the 90%
saturated test solution (based on analysis of dissolved organic carbon
in the test solutions) - Results of TOC analyses
by elemental analysis, performed under non-GLP conditions
As the DOC values of replicate one
(after 72 h) and replicate two (after seven days) were significantly
different an additional DOC measurement of replicate two was performed
resulting in a concentration, which confirmed the first result obtained
on replicate two.
surface tension of an aqueous solution of test item was determined
according to EU method A.5 and OECD 115 under
GLP using the plate method.
Two 90% saturated test solutions were
prepared (replicate one and replicate two). They are obtained from
aqueous solutions of test item prepared with a loading rate of 1.00 g
test item/L, shaked for 72h (replicate one) and for seven days
The determination of surface tension
(and verification) was conducted on each replicate.
The first determination was determined
on replicate one, as 66.39 ± 0.16 mN/m at 20.5 °C. After 61 minutes, the
verification was performed. No significant changes and thus no
significant dependence on time was observed.
The second determination was
determined on replicate two as 68.28 ± 0.26 mN/m at 20.3 °C. After 65
minutes, the verification was performed. No significant changes and thus
no significant dependence on time was observed.
The difference of surface tension
between the two determinations (after 72h and after seven days),
calculated as 2.85%, laid within the criterion validity of 5%.
Therefore, no significant dependence on time was observed.
As these values were higher than 60
mN/m, the test item was classified as not surface active substance.
The surface tension of the negative
control laid within the accepted range.
Note that the concentration of the
test item in the 90% saturated test solutions was determined (based on
analysis of dissolved organic carbon in the test solution) to be 49.32
mg test item/L for the replicate one and 15.49 mg test item/L for the
replicate two. This latest value was confirmed by an additional DOC
measurement resulting in a concentration of 14.70 mg test item/L.
Considering the significant difference
between concentrations estimated in replicate one and two, no key value
for concentration of the test solution has been retained.
Only a range of estimated
concentrations can be stated : between 14.70 and 49.32 mg of test item/L
This is considered as not critical
considering that a similar surface tension was obtained for both test
The surface tension of aqueous solutions of
the test item was determined as 67.33 mN/m at 20.4 °C (mean of two
determinations obtained from two 90% saturated solutions prepared with a
loading rate of 1g/L of test item and two shaking times to saturate the
solution of 72h and 7 days respectively)
Concentration of test solutions : between
14.70 and 49.32 mg of test item/L (estimated based on analysis of
dissolved organic carbon in the test solutions).
According to EU A5 criteria, as the result
is above the threshold value of 60 mN/m, the substance is considered to
have NO surface-active potential under the experimental conditions.
A fully reliable
experimental study, conducted according to a recognized OECD/EU method
(plate method) and under GLP, is available.
It is considered as a key study.
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