Lupine, L. albus, ext.

Melting point / freezing point

Link to relevant study record(s)

Reference

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 April to 13 May 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised with its purity. Therefore full validation applies.

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

EU Method A.1 (Melting / Freezing Temperature)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP Compliance Programme (inspected on December 22 and 23 2014/ signed on July 28, 2015)

Type of method:

differential scanning calorimetry

Key result

Atm. press.:

>= 101 320 - <= 101 330 Pa

Decomposition:

yes

Decomp. temp.:

> 220 - < 300 °C

Remarks on result:

other: No melting point was recorded, the test item inflated and darkened

Remarks:

Mass of test item 4.24 mg Program Phase 1: Isotherm at about 25 °C for 5 min Heating phase from 25 °C to 600 °C at 10 °C/min

No melting point was recorded, the test item inflated and darkened. For further information, please see the graphs 1, 2 and 3 in the report.

Three assays were perfomed

Here below the conditions of each test:

Assay No. 1

Gas used: Nitrogen

Ventilation: Yes

Crucible used: Aluminium, 120 µL, with lid

Mass of test item: 4.24 mg

Program: Phase 1: Isotherm at about 25 °C for 5 min

Heating phase from 25 °C to 600 °C at 10 °C/min No melting point was recorded. When heating, the test item inflated and darkened. It was recorded as an exothermic reaction between about 220 °C and 300 °C.

Assay No. 2

Gas used: Nitrogen

Ventilation: Yes

Crucible used: Aluminium, 120 µL, with lid

Mass of test item: 10.23 mg

Program: Phase 1: Isotherm at about 25 °C for 5 min Heating phase from 25 °C to 600 °C at 10 °C/min

More test item was weighed to confirm the reaction and no melting point was recorded but a strong baseline jump which did not give any information. A third assay was made with a smaller quantity to eventually highlight a reaction.

Assay No. 3

Gas used: Nitrogen

Ventilation: Yes

Crucible used: Aluminium, 120 µL, with lid

Mass of test item: 1.74 mg

Program: Phase 1: Isotherm at about 25 °C for 5 min

Heating phase from 25 °C to 600 °C at 10 °C/min

With a smaller quantity, no melting point was recorded. When heating, the test item inflated and darkened. It was recorded as an exothermic reaction at about 300 °C.

Conclusions:

No melting point was recorded.

Executive summary:

The melting point of the test substance was tested under GLP according to EU A1 and OECD 102, by differential scanning calorimetry. Three runs were performed under nitrogen atmosphere, and no melting point was recorded.

The test item inflated and darkened sign of decomposition.

Description of key information

No melting point was recorded under nitrogen atmosphere.

Additional information

A fully reliable experimental study, conducted according to a recognized EU/OECD method and under GLP, is available. No melting point was recorded, the test item was inflated and darkened.