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EC number: 203-131-3
CAS number: 103-64-0
The Adsorption Coefficient of test substance
was determined as per the HPLC method (OECD Guideline-121). The Log Koc
value was determined to be 3.263 ± 0.001 at 25°C. Thus based on the
result it is concluded that the test substance has a moderate sorption
to soil and sediment and therefore has slow migration potential to
The adsorption coefficient Koc in soil and
in sewage sludge of test chemical was determined by the Reverse Phase
High Performance Liquid Chromatographic method according to OECD
Guideline No. 121 for testing of Chemicals. The solutions of the test
substance and reference substances were prepared in appropriate solvents.
A test item solution was prepared by
accurately pipetting 4 microlitre of test item and diluted with
Acetonitrile up to 10 ml. Thus, the test solution concentration was
570.8 mg/l(calculated according to its density). The pH of test
substance was 4.4. Each of the reference substance and test substance
were analysed by HPLC at 210 nm. After equilibration of the HPLC system,
Urea was injected first, the reference substances were injected in
duplicate, followed by the test chemical solution in duplicate.
Reference substances were injected again after test sample, no change in
retention time of reference substances was observed. Retention time tR
were measured, averaged and the decimal logarithms of the capacity
factors k were calculated. The graph was plotted between log Koc versus
log k(Annex - 2).The linear regression parameter of the relationship log
Koc vs log k were also calculated from the data obtained with
calibration samples and therewith, log Koc of the test substance was
determined from its measured capacity factor. The reference substances
4-chloroaniline, N-methylaniline, p-toluamide, Aniline,
2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol,
3-nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, 1-naphthylamine,
1-naphtol, Direct Red 81, Benzoic acid methylester,
Carbendazim, Benzoic acid phenylester, Xylene, Ethylbenzene, Toluene,
Naphthalene, 1,2,3-trichlorobenzene, Pentachlorophenol, Phenol,
N,N-dimethylbenzamide, 3,5- dinitrobenzamide, N-methylbenzamide,
Benzamide, DDT, Acetanilide were chosen having Koc value range from 1.25
The Log Koc value of test chemical was
determined to be 3.263 ± 0.001 dimensionless at 25°C.This log Koc value
indicates that the substance has a moderate sorption to soil and
sediment and therefore has slow migration potential to ground water.
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