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EC number: 232-077-3
CAS number: 7785-26-4
Table 6.1.3/2: Acute immobilization of
daphnids after 24 and 48 hours in the final test
Nominal concentration (mg/L)
Number of daphnids exposed
Response at 24 h
Response at 48 h
An acute aquatic toxicity study was
performed on Daphnia magna with (-)-alpha-pinene in accordance
with GLP and OECD Guideline 202. Following a preliminary range-finding
test and a previous test, twenty daphnids (four replicates, five
daphnids/replicate) were exposed to an aqueous solution of the test
item, (-)-alpha pinene, at the required nominal test concentrations of
0.2, 0.3, 0.4, 0.7, 1.0 and 1.5 mg/L. The immobility of the daphnids was
determined in a semi-static 48-h test by visual observation after 24 and
The concentrations of the test item were
determined by chemical analyses at the start (t = 0 h), at t = 24 h (new
and old solutions) and at the end of the test (t = 48 h). The test item
levels were found to be not stable throughout the test. Indeed,
concentrations measured in freshly prepared solutions and in 24-h old
solutions revealed that the concentration of the test item was not
satisfactorily maintained within ± 20 % of the initial concentration
throughout the test. Thus, the evaluation of the effects on Daphnia
magna was based on the geometric means of the measured
concentrations: 0.156, 0.242, 0.361, 0.511, 0.685 and 1.190 mg/L.
After 24 h of exposure, immobilizations were
0, 0, 0, 0, 0, 25 and 50 % at 0, 0.156, 0.242, 0.361, 0.511, 0.685 and
1.190 mg/L, respectively. After 48 h of exposure, immobilizations were
0, 0, 35, 40, 35, 60 and 100 % at the respective concentrations. The
EC50 values at 24 and 48 h were 1.124 (0.934-1.598) and 0.475
(0.248-1.132) mg/L respectively, based on the average of measured
48h-EC50 for freshwater invertebrates of (-)-alpha-pinene was 0.475 mg/L.
One reliable study is available for the
substance itself and is selected as key study. The toxicity of (-)-alpha
pinene to freshwater Daphnia magna was studied according to OECD
202 and GLP. Daphnids were exposed to substance at nominal test
concentrations of 0.2, 0.3, 0.4, 0.7, 1.0 and 1.5 mg/L over a test
period of 48 hours (semi-static test, daily renewal, closed conditions).
Concentrations were analytically verified at the start and daily until
the end of the test (old and new test media). The concentration of the
test item was not satisfactorily maintained within ± 20% of the initial
concentration throughout the test. Thus, the endpoint was based on the
geometric means of the measured concentrations. The 48h-EC50 was
determined to be 0.475 mg/L, the EC0 and EC100 were 0.156 mg/L and 1.190
the 48h-EC50 is valid for key value for chemical safety assessment.
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