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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

toxicity to microorganisms, other
Ready biodegradability test according to OECD 301 D used to derive PNEC microorganisms.
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
read-across: supporting information
according to guideline
other: OECD 301 D
Principles of method if other than guideline:
See cross-referenced study
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
Determinations of the concentration of dissolved oxygen using an oxygen electrode in duplicate vessels
were made at day 0, 7, 14, 21, 29, 35, 42, 49 and 56 for the “test suspension” and “inoculum blank with
silica gel” series, at day 0, 7, 14, 21 and 29 for the “procedure control” and “inoculum blank without silica
gel” series, and only at the start of the test and after 7 and 14 days of incubation for the “toxicity control”
Temperature: Measured continuously in the growth chamber
Details on test solutions:
See cross-referenced study
Test organisms (species):
aerobic microorganisms
Details on inoculum:
Origin: River water was sampled from “L’Adour”, situated downstream of a plant treating domestic was
tewater (Aureilhan, France).
Pre-conditioning: The river water was aerated for about 7 days before use as inoculum at the test
temperature to reduce endogenous respiration.
Test type:
Water media type:
Limit test:
Total exposure duration:
28 d
Post exposure observation period:
No data
Test temperature:
between 20.6 and 21.3°C throughout the test
no data
Not applicable
No data
Nominal and measured concentrations:
Nominal concentration: 2 mg/L
Details on test conditions:
See cross-referenced study
Reference substance (positive control):
Key result
28 d
Dose descriptor:
Effect conc.:
ca. 2 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
other: readily biodegradable at this nominal test concentration
Details on results:
Reported statistics and error estimates:
Validity criteria fulfilled:
The test item is considered as readily biodegradable and the tested concentration is taken as NOECmicrooorganism.
Executive summary:

A ready biodegradability study was performed according to OECD Guideline for Testing of Chemicals No. 301 D, "Ready biodegradability – Closed Bottle Test" (1), referenced as Method C.4-E of Commission Regulation No. 440/2008 amended by Commission Regulation (EU) 2016/266 (2) and with ISO 10634 (7).

Ten BOD bottles were filled with mineral medium inoculated with river water and test item added at a nominal concentration of 2 mg/L with the help of silica gel.

Therefore, the tested concentration of 2 mg/L can be taken as conservative NOECmicrorganism.

Description of key information

The tested concentration in the ready biodegradability study (2 mg/L) conducted with alpha-pinene multiconstituent is taken as conservative NOECmicrorganism.

Key value for chemical safety assessment

EC50 for microorganisms:
2 mg/L

Additional information

A ready biodegradability study was performed on alpha-pinene multiconstituent according to OECD Guideline for Testing of Chemicals No. 301 D.

The tested concentration of 2 mg/L is taken as conservative NOECmicrorganism and is valid for key value for chemical safety assessment.