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EC number: 232-077-3
CAS number: 7785-26-4
Negative control (PBS+): mean OD = 0.821
Positive control (5% SDS): % viability (MTT
assay) = 18.7 ±
Test item: % viability (MTT assay) = 39.6 ±
Table 1: MTT conversion assay in
Mean Viability %
A GLP study conducted in vitro with human
epidermis model EPISKIN was performed to assess the irritancy potential
of alpha-pinene similarly to ECVAM protocol version 1.8 of February
2009. 10 µL of test item was applied directly on epidermis for 15 min on
3 epidermis. Positive control was 10 µL of 5% (w/v) SDS solution and
negative control was 10 µL of PBS (each tested on 3 epidermis). MTT
conversion assay was peformed to evaluate the percentage of cellular
viability of the epidermis. Positive control had a percentage of cell
viability of 18.7 ± 3.0 and test item 39.6 ± 5.6. As the percentage of
viability is ≤ 50 %, the test item is considered to be irritating for
Therefore, alpha-pinene is classified as
irritating to skin, R38, according to the criteria of Directive
67/548/EEC and in category 2 according to Regulation (EC) n° 1272/2008
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