N-[3-({[2,2-dimethyl-3-(morpholin-4-yl)propylidene]amino}methyl)-3,5,5-trimethylcyclohexyl]-2,2-dimethyl-3-(morpholin-4-yl)propan-1-imine

Endpoint summary

Additional information

Acute toxicity to fish:

In a 96-h Toxicity Test with Zebrafish the acute toxicity of the test item on fish was evaluated. A limit test was performed in which the test animals were exposed to aqueous test media containing the test item for 96 hours at one concentration (100 mg/L) plus a control. Based on the results of an analytical method validation the concentration of the test item was expected to remain within 80 - 120 % of the nominal over the testing period, therefore, the experiment was carried out under static conditions. The measured test item concentration remained within ± 20 % of the nominal concentrations over the test period of 96 hours; therefore, the biological results are based on the nominal concentration. Mortality and any sub-lethal effects were not observed during the 96-h exposure period either in the treated or in the control group. Accordingly, the 96-h NOEC was determined to be 100 mg/L and the LOEC and the LC50 values were determined to be higher than 100 mg/L.

 

Toxicity to aquatic invertebrates:

In a 48-hour acute toxicity test with Daphnia magna the 48-h EC50 value was determined to be 40.2 mg/L, and the 48-h NOEC was determined to be 17.1 mg/L. These results are based on the nominal test item concentrations.

 

Toxicity to aquatic algae:

In a 72-h algal growth inhibition test with Pseudokirchneriella subcapitata, the 72-h EC50 value based on growth rate was determined as 89.0 mg/L and based on yield was determined as 9.4 mg/L. The 72-h EC10 value based on growth rate was determined as 3.8 mg/L and based on yield was determined as 0.5 mg/L. The 72-h NOEC related to growth rate was determined to be 2.4 mg/L and related to yield was determined to be 1.0 mg/L.

 

Activated sludge respiration inhibition test:

The purpose of the 3-hour test was to evaluate the influence of the test item Sika Hardener MI (Sika Härter MI) on the activity of the activated sludge by measuring the respiration rate under defined conditions The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours. Based on the preliminary information about the test item caused effect on the activated sludge inoculum, the test item Sika Hardener MI (Sika Härter MI) was investigated at the concentration of 1000 mg/L as a limit concentration, only. Defined amounts of the test item were added directly into the test vessels. In parallel with the test item treatments 3,5-Dichlorophenol as positive reference control in a concentrations of 2, 7 and 24.5 mg/L; furthermore blank (inoculum) control and nitrification controls were investigated. The main test was performed without abiotic controls, based on the results of the preliminary test where abiotic controls were tested at the test item concentration of 1000 mg/L and no remarkable abiotic oxygen consumption was noticed. The test was performed without pH adjustment. The test item was investigated at one (limit) concentration level of 1000 mg/L. The observed oxygen consumption rates consequently the specific respiration rates were in the range of the blank controls, no inhibitory effect of the test item was observed. Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are higher than 1000 mg/L. The specific respiration rates were compared with the blank control values using 2 Sample t-Test (a=0.05). No statistical significant differences were observed in the comparison with the blank control values, consequently based on the results of this study the NOEC can be statistically and biologically determined as ≥ 1000 mg/L.