ethyl 5,5-diphenyl-2-isoxazoline-3-carboxylate

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 Jun 1998 - 08 Jul 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

adopted 1984

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

For the preparation of the test substrate 740 g basic substrate for each replicate were filled into a stainless steel vessel. Each replicate for each concentration was mixed separately. Calculations of the soil concentrations of the test substance were based on 100% purity of the test material.
To obtain fresh stock mixture for the test, the test substance was weighed out to a precision of 0.1 mg and mixed in industrial sand. 10 g stock mixture were added to the basic substrate in the mixing vessel and mixed thoroughly with a rapid mixer (740 g basic substrate + 10 g stock mixture = 750 g test substrate).

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Subspecies: Eisenia fetida andrei
- Common name: Earth worm
- Source and breeding: The test organisms were bred in the animal maintenance room of the Ecobiology of AgrEvo in plastic boxes in a mixture of 4 parts sphagnum peat (Neuhaus Düngetorf, grade H2-H8, Torfwerke Neuhaus GmbH, Saterland, Germany), 4 parts dried and pulverised cow manure (Tierhaltung, H813, Hoechst AG, Germany), and 1 part of straw (animal holding facility of Hoechst AG, Germany) in accordance with the OECD guidelines. The approximate values of internal dimensions of the boxes are 440 mm long x 220 mm wide x 210 mm high. About 300 worms are cultured per box. The relative air-humidity in the room was approximately 50%. The breeding temperature was 20 ± 2 °C. The room was illuminated by wide spectrum fluorescent tubes during a 16 h daylight photoperiod. The moisture content in the breeding boxes was kept between 70 and 80%. Twice monthly pH, temperature and moisture content were determined from every culturing box. If necessary, the breeding substrate was moistened with deionised water. The substrate was renewed quarterly.
- Age at test initiation: > 2 months with distinct clitellum
- Weight at test initiation: 4.60 - 5.24 g/10 worms

ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): not reported

Study type:

laboratory study

Substrate type:

artificial soil

Total exposure duration:

14 d

Test temperature:

20 ± 2 °C

pH:

5.9 - 6.3

Moisture:

test start: 25.48%
test end: 25.06%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1,5 L glass jars, diameter 10 cm, height 20 cm
- Test container closed: yes, with glass lids
- Amount of soil or substrate: 750 g of test substrate
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- Biomass loading rate: 4.60 - 5.24 g per glass jar

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 10% sphagnum peat, 20% kaolin clay, 70% industrial sand
- Pretreatment of soil: For adjustment of pH, 0.4% CaCO3 was added to basic soil substrate
- Storage (condition, duration): Basic artificial soil substrate was freshly prepared

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, cocoon production and intoxication symptoms after 7 and 14 days

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Nominal and measured concentrations:

Nominal: 100, 180, 320, 560, 1000 mg/kg substrate (dry weight)

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

560 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: body weight

Details on results:

- Mortality at end of exposure period: no mortality and no intoxication symptoms after 7 and 14 days
- Total mass of adults at beginning of test: 4.93 - 5.04 g
- Reduction of body weight of live adults (% of initial weight) at end of exposure period: 4.5 - 17.4%

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: LC50 after 7 and 14 days: 25 mg/kg soil dw; NOEC 18 mg/kg soil dw

Reported statistics and error estimates:

Body weight loss of 17.4% of initial weight after 14 d in the group treated with 1000 mg/kg test substance was significantly different from the control (α = 0.05). No significant difference between the other groups showing 4.46 - 8.08% weight loss and the control was observed/calculated.

Validity criteria:

Mortality at end of exposure period: no mortality and no intoxication symptoms after 14 days (should be < 10%).

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.

Description of key information

LC50 (14 d): > 1000 mg/kg soil dw (nominal, Eisenia fetida, OECD 207)

NOEC (14 d): 560 mg/kg soil dw (nominal, Eisenia fetida, OECD 207)

Additional information

The toxicity towards soil organisms was investigated in a 14-day artificial soil test with the earthworm Eisenia fetida following OECD guideline 207. The 14 d LC 50 was > 1000 mg/kg soil dry weight. The NOEC of the test substance to the weight of earthworms (Eisenia fetida) was found to be 560 mg/kg soil dry weight.