Registration Dossier
Registration Dossier
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EC number: 424-660-8 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 5-11 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: done under GLP and OECD method
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 424-660-8
- EC Name:
- -
- Cas Number:
- 224631-15-6
- Molecular formula:
- Hill formula: C18H26N4O5S CAS formula: C18H26N4O5S
- IUPAC Name:
- 2,5-dioxopyrrolidin-1-yl (2S)-3-methyl-2-{[methyl({[2-(propan-2-yl)-1,3-thiazol-4-yl]methyl})carbamoyl]amino}butanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Chemical anlaysis was completed on samples to confirm concentrations. HPLC was used.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A 100 mg/l "aged" stock slution of A-133816.0 was prepared by adding a weighed amount of A-1338163.0 (0.498g) to a 5L volumettric flask and bringing the flask to volume with Daphnia medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Fill in what you can
TEST ORGANISM
- Common name: water flea
- Strain: 278/4
- Source: CCAP, Ambleside, Cumbria
- Age at study initiation (mean and range, SD):
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Those animals which were unable to swim within 15 s following gentle agitation of the test contianer were considered immobile. No immoblie Daphnia were recorded during the study.
Test conditions
- Hardness:
- 164 mg CaCO3 mg/l
- Test temperature:
- 19.6-20.3 oC
- pH:
- pH was monitored
- Nominal and measured concentrations:
- Nominal concentration = 100 mg/l "aged solutuon"
Chemical analysis was conducted on the "aged" solutions to confirm that A-133816.0 had degraded to 2,4-wing acid. - Details on test conditions:
- Test vessel: glass crystallising dishes
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 200 ml capacity
- Aeration: ventilation holes
- Renewal rate of test solution (frequency/flow rate):
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates):
OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 hour dark. Illumination provided by artificial daylight fluroescent tubes
TEST CONCENTRATIONS
- Range finding study: Basedon the results of hydrolysis studies conducted under INveresk Project No. 374979, a limit test was conducted on an "aged" solution of 100 mg/l nominal A-133816.0
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for immobilisation and NOEC are >100 mg/l nomial "aged" A-133816.0
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