Octanoic acid, 2-phenoxyethyl ester

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 April - 2 May 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: from freshly prepared medium and from aged medium after 24 hours

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A water soluble fraction (WSF) was prepared. The test item was dispersed in test water and the dispersion was homogenized with an ultraturrax 15 minutes with 17000 rpm at room temperature.Subsequently the undissolved particles were removed by membrane filtration (membrane filter 0.45 μm, RC).
The filter was saturated in order to avoid adsorption during the filtration. To achieve this, the first 50 ml filtrate were discarded. Then, the filtration was interrupted for ca. 15 minutes to allow adsorption and saturation of the filter material with dissolved test item. Thereafter, the filtration was continued. The next 50 ml were discarded. During filtration the filter was always be kept covered. The following filtrate, i.e. the water soluble fraction, was used in the test. This preparation procedure was carried out for each test media renewal.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: All fish used in the test was gained at Noack Laboratorien GmbH from a single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin)
-- Maintenance of the brood fish: Holding was performed at the test facility at 23 ± 2 °C and diffuse light (7 - 750 Lux, natural photoperiod). The water was changed at least once per week.

ACCLIMATION
- Acclimation period: 12 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: Sera Vipan (SERA GMBH, Heinsberg, Germany)
- Feeding frequency during acclimation: 3 times per week, 4 % of the fish body weight per feeding day. The test fish were not be fed 24 h before test start.
- Health during acclimation (any mortality observed): no mortality observed

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

60 mg/L as CaCQ3

Test temperature:

22.2 - 23.0 °C

pH:

7.44 - 7.81

Dissolved oxygen:

90-100% saturation

Nominal and measured concentrations:

Nominal test substance concentration: 100 mg/L (WSF)
Measured test substance concentration: at test start: 1.12 mg/L (1.1% of nominal), after 24 h: 0.517 mg/L (0.5% of nominal)
Geometric mean measured concentration: 0.340 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria (13 L)
- Type (delete if not applicable): loosely covered with a glass lid.
- Size, fill volume: 25.5/18/28 cm, 10 L
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): every 24 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):2
- Biomass loading rate: 0.07 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: 7 - 750 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality or symptoms of intoxication after 2, 24, 48, 72 and 96 h

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 0.34 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no

Sublethal observations / clinical signs:

Cumulative Mortality [%] in the Test Vessels

Geometric mean measured concentration [mg/L]	Cumulative mortality at observation time [hours
	2	24	48	72	96
0.340	0	0	0	0	0
Control	0	0	0	0	0

Description of key information

No toxic effects on fish up to the limit of water solubility.

Additional information

The acute toxicity of 2-Phenoxyethyl octanoate (CAS 23511-73-1) to fish was tested in a semi-static limit test according to OECD 203. Zebra fish (Danio rerio) were exposed to a nominal test substance concentration of 100 mg/L (nominal, WSF) for 96 hours. The test medium was renewed every 24 hours. The geometric mean measured test substance concentration was 0.34 mg/L. The mortality of the test fish was determined after 2, 24, 48, 72 and 96 hours. Mortality or symptoms of intoxication were not observed. The reported LL50(96h) value is > 0.34 mg/L (geom. mean, WSF).

Based on the available acute toxicity data toxic effects of the substance 2-Phenoxyethyl octanoate are not expected.