5-acetyl-1,3-phenylene diacetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 January 1999 - 18 June 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and according to an OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,5-Diacetoxyacetophenon

IUPAC Name:

3,5-Diacetoxyacetophenon

Test material form:

other: solid

Details on test material:

- Physical state: Brownish solid
- Analytical purity: Not indicated by sponsor; treated as 100% pure
- Lot/batch No.: 286
- Expiration date of the lot/batch: 16 December 1999
- Storage condition of test material: At room temperature in the dark
- Other:

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

During the final test samples were taken from 10, 32 and 100 mg/1 for analysis. Also samples
were taken from the controls.
Sampling: Frequency: at t= 0 h from freshly prepared solutions and at t= 24 h
from the freshly prepared solutions and the 24h-old solutions.
Volume: 10 ml from the approximate centre of the lest vessel.
Storage: Not applicable, samples were analysed on the day of sampling.

Additionally, reserve samples of 10 ml were taken from all concentrations. These samples were
stored in a deep-freeze for possible analysis until delivery of the final report with a maximum of
three months. Specification of the samples analysed and the method of analysis are described
in the appended Analytical Report.

Test solutions

Vehicle:

no

Details on test solutions:

Basedon the results of the range-finding test, a range of
nominal concentrations from 10 to 100 mg/1 increasing by
a factor of 1 .8.

Preparation during the static range-finding test started with a stock solution at 100 mg/l.
Treatment with ultrasonic waves was applied to dissolve the test substance in the test medium.
Thereafter, the stock solution was cooled to approximately 20 °C. The lower test concentraticns
were prepared by subsequent dilutions of this stock solution in test medium. Test solutions for
the semi-static final study were prepared similarly. However, test solutions were renewed after
24 hours of exposure. The final test solutions were all clear and colourless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: (Crustacea, Cladocera) (Straus, 1820)
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding:
Start of each batch: With new-born animals. i.e. less than 3 days old, by
placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature of medium: 18-22°C, constant within ± 1°C
Feeding: Daily, a suspension of fresh water algae.
Medium: M7, as prescribed by Dr. Elendt-Schneider (Eiendt, B.P.,
1990: Selenium deficiency in Crustacea. An
ultrastructural approach to antennal darnage in
Daphnia magna Straus. Protoplasma 154, 25-33).
- Feeding during test: no

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 to 20.3°C

pH:

7.4 - 8.0

Dissolved oxygen:

7.5 - 9.0

Nominal and measured concentrations:

norminal: 10, 18, 32, 56 and 100 mg/l
measured 5.7; 11.7, 22.4, 41.8 and 76.6 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml, all-glass
- fill volume: 80 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): after 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO, prepared in milli-RO water

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

14 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

The actual responses in this reference test with K2Cr207 are within the ranges of the expected
responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was
in agreement with the historical data collected at NOTOX.
The 24h-EC50 was > 1.8 mg/l.
The 48h-EC50 was 0.95 mg/l with 95% fiducial limits of 0.78- 1.3 mg/l.

Any other information on results incl. tables

ACCEPTABILITY OF THE TEST

1. ln the controls, no daphnia became immobilised or trapped at the surface of the water.

2. The analytical program showed that the actual test concentrations could not be maintained at 80% of the initial concentration, despite renewal of the test solutions after 24 hours of exposure. Hence, determination of the EC50-values was based on the average exposure concentrations, i.e. the mean of the concentrations measured at the start and after 24 hours of exposure.

3. Further, all test conditions remained within the ranges prescribed by the protocol.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 values were based on the average exposure concentrations expressed as mg/l.
Under the conditions of the present study, the EC50 values for mobility of Daphnia magna
exposed to 3,5-DIACETOXYACETOPHENON were as follows:
The 24h-EC50 was 89 mg/1 with a 95% confidence interval between 73 and 130 mg/1.
The 48h-EC50 was 14 mg/1 with a 95% confidence interval between 11 and 18 mg/1.