5-acetyl-1,3-phenylene diacetate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 13, 1999 - April 21, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted in GLP compliance and according to an OECD guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,5-Diacetoxyacetophenon

IUPAC Name:

3,5-Diacetoxyacetophenon

Test material form:

other: solid

Details on test material:

- Physical state: Brownish solid
- Analytical purity: Not indicated by sponsor; treated as 100% pure
- Lot/batch No.: 286
- Expiration date of the lot/batch: 16 December 1999
- Storage condition of test material: At room temperature in the dark
- Other:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: Waterschap de Maaskant', 's-Hertogenbosch, Netherlands

The sludge was kept under continuous aeration until
further treatment. The concentration of suspended
solids was 1.0 g/l in the concentrated sludge
(information obtained from the municipal sewage
treatment plant). Before use, the sludge was allowed
to settle for at least 30 minutes and the liquid
decanted for use as inoculum. Since the amount of
suspended solids was very low the amount of the
liquid for use as inoculum was increased to 40 ml/l of
mineral medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Test performance:

The results of the biodegradation test were considered to be valid when:
the total C02 evolution in the inoculum blank at the end of the test did not normally
exceed 40 mg/l. lf values greater than 70 mg C02/I are obtained, the data and
experimental technique should be examined critically.
For the calculation of the total C02 evolution in the inoculum blank the volume of HCI
needed for the titrating of the remaining Ba(OH)2 of the inoculum blanks and for fresh
Ba(OH)2 was used.
the difference of duplicate values for the %-degradation of the test substance at the
plateau, at the end of the test or at the end of the 10-day window, as appropriate, was
less than 20.
the percentage degradation of the reference substance reached the Ievel for ready
biodegradability (60%) by 14 days.
Because of the stringency of the method, low values do not necessarily mean that the test
substance is not biodegradable under environmental conditions, but indicates more work will be
necessary to establish biodegradability.

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

30 - 48

Sampling time:

28 d

Remarks on result:

other: bottle 1

Details on results:

The relative degradation values calculated from the measurements performed during the test
period revealed 30 and 48% degradation of 3,5-DIACETOXYACETOPHENON for A and B
respectively. Thus, the criterion for ready biodegradability (at least 60% degradation within 10
days of biodegradation exceeding 10%) was not met.
in the toxicity controlless than 25% degradation occurred within 14 days (based on ThC02 ).
Therefore, the test substance was found to have an inhibiting effect on microbial activity

BOD5 / COD results

Results with reference substance:

The positive control substance was degraded at least 60% within 10 days.
The total C02 release in the blank reached a total value of 37 mg C02 per 2 litres of medium.
The difference of duplicate values for %-degradation of 3,5-DIACETOXYACETOPHENON was
always less than 20.
Since all criteria for acceptability of the test were met, this study was considered to be valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

3,5-DIACETOXYACETOPHENON was not readily biodegradable under the conditions of the
modified Sturm test presently performed.
However, it should be noted that in the toxicity control 3,5-DIACETOXYACETOPHENON was
found to have an inhibiting effect on microbial activity.