Esterification products of dihydrofuran-2,5-dione, C16-24 (even numbered) alkenyl with propane-1,2,3-triol and propane-1,2,3-triol oligomers

Short-term toxicity to fish

Description of key information

Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave an LC50 value of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.

Additional information

A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.

In accordance with the recommendations of REACh, the test was conducted according to the threshold approach recommended by ECHA. Using this approach the lowest EC50 value from either the Algal Growth Inhibition test or Acute Toxicity to Daphnia magna study is set as the threshold concentration and a "Limit test" is conducted at this threshold concentration. If no mortalities are observed this indicates that fish are not the most sensitive species and that the LC50 is greater than the threshold concentration. Therefore, as the EC50 values obtained for both the Acute Toxicity to Daphnia magna study and the Algal Growth Inhibition study (Harlan Study Numbers 41204772 and 41204767 respectively) were greater than 100 mg/L, the test was conducted at a single concentration of 100 mg/L to ensure that toxicity was not observed at this concentration.

Seven fish were exposed to an aqueous dispersion of the test item, at a single concentration of 100 mg/L for a period of 96 hours at a temperature of approximately 14 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

Analysis of the test preparations at 0 and 72 hours (fresh media) and at 24 and 96 hours (old media) showed measured test concentrations to range from 96% to 100% of nominal and so the results are based on nominal test concentrations only. Exposure of rainbow trout (Oncorhynchus mykiss) to the test item gave an LC50 value of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L. It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/L.