C.I. Reactive Yellow 161

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 - 14 Jul 1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1981

Deviations:

yes

Remarks:

compared to the newer versions of the guideline, only 24 h were tested in the version of 1981

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

Samples for analysis were taken at 0 h and 24 h exposure

Vehicle:

no

Details on test solutions:

1000 mg of the test item were dissolved in water and made up to 1000 mL.
Calculated amounts of stock solution to produce the desired test concentrations were given into the water and homogeneously distributed.

Test organisms (species):

Daphnia magna

Details on test organisms:

Breeding: parthenogenetic reproduction
Feeding: with a suspension of green algae and yeast
Collection: 24 hours before the start of the test reproductive daphnia are separated from the young by sieving through a 800 µm sieve.This operation is repeated immediately before the start and the young daphnia (0 - 24h old) are retained for the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

19 - 22°C

pH:

7.6 - 7.9

Dissolved oxygen:

8.0 - 8.5 mg/L

Nominal and measured concentrations:

0 (control), 100, 180, 320, 580, 1000 mg/L nominal
0 (control) , 91, 162, 299, 549, 965 mg/L measured (24 h)

Details on test conditions:

TEST SYSTEM
- Test vessel: tubes
- Material, size, headspace, fill volume: plastic, 20 mL volume (10 mL fill volume), closed with steel cap
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconsitituted water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 0 and 24 h

Reference substance (positive control):

no

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: analytically verified

Details on results:

24-h EC0 = 320 mg/L
Mortality in control 0%
Precipitation occurred in test concentrations >100 mg/L

Reported statistics and error estimates:

N/A

Validity criteria fulfilled:

yes

Conclusions:

The substance has a 24-EC50 > 1000 mg/L to Daphnia magna (analytically verified). The lowest effect value (EC0) was determined at 320 mg/L. Reactive Yellow 161 is acutely not harmful to aquatic invertebrates.

Description of key information

The substance has a 24-EC50 > 1000 mg/L to Daphnia magna (analytically verified). The lowest effect value (EC0) was determined at 320 mg/L. Reactive Yellow 161 is acutely not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 000 mg/L