C.I. Reactive Yellow 161

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 - 25 Nov 1983

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

May 1981

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the bath at the beginning and at the end of the experiment

Vehicle:

no

Details on test solutions:

Direct weighing of 3 g substance in 3 L diluent (reconstituted water according to ISO 7346), stirring until complete dilution

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West-Aquarium, Bad Lauterberg, Germany
- Age at study initiation: 4.5 months
- Length at study initiation (mean): 2.8 cm
- Weight at study initiation (mean): 0.27 g

ACCLIMATION
- Acclimation period: 46 d in dechlorinated tap water
- Acclimation conditions (same as test or not): same except medium

FEEDING DURING TEST
None

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

100 mg/L (CaCO3)

Test temperature:

23.0 - 24.5°C

pH:

7.5 - 7.8

Dissolved oxygen:

7.4 - 8.2 mg/L

Nominal and measured concentrations:

0 (control), 1000 mg/L nominal
0 (control), 1030 (initial) - 1056 (end) mg/L measured corresponding to 103 - 106% of nominal concentration

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 5 L (3 L fill volume)
- Aeration: slowly
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 animal / 300 mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Culture medium different from test medium: yes, dechlorinated tap water vs. reconstituted water (ISO 7346)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 h
- Light intensity: yellow light TL16 / 20W

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 3, 24, 48, 72 and 96h after start of exposure

TEST CONCENTRATIONS
- 1000 mg/L limit test
- Range finding study
- Test concentrations: 100, 300 and 1000 mg/L
- Results used to determine the conditions for the definitive study: yes, no mortality occurred in pre-test

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: analytically verified

Details on results:

0% mortality in test concentration and negative control
LC0 > 1000 mg/L

Results with reference substance (positive control):

N/A

Reported statistics and error estimates:

None

Validity criteria fulfilled:

yes

Conclusions:

The substance has a 96-h LC50 > 1000 mg/L to fish (analytically verified). Reactive Yellow 161 did not cause any lethal effects up to 1000 mg/L in an OECD 203 study with Danio rerio.

Description of key information

The substance has a 96-h LC50 > 1000 mg/L to fish (analytically verified). Reactive Yellow 161 did not cause any lethal effects up to 1000 mg/L in an OECD 203 study with Danio rerio.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L