Pentene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 May 1993 to 26 November 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standrd test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of inspection: 17/08/1992 Date of signature: 30/10/1992

Test material

Test material information

Constituent 1

Reference substance name:

Pentene

EC Number:

246-916-6

EC Name:

Pentene

Cas Number:

25377-72-4

Molecular formula:

C5H10

IUPAC Name:

pent-1-ene

Details on test material:

- Name of test material (as cited in study report): Pentene-1
- Substance type: Organic
- Physical state: Liquid
- Analytical purity: 98.4% m/m
- Impurities (identity and concentrations):
n-Pentene and Iso-pentene - 0.33 % m/m
2-MB-1 and 2-MB-2 - 0.93% m/m
Cis and tans pentene-2 - 0.1% m/m
Other unsaturated C5-hydrocarbons: 0.13% m/m
Non-volatile residue: 0.001% m/m
- Purity test date: 23 September 1992
- Lot/batch No.: Tank 1814, Indent No. 9200/9061-01, code no. X8279
- Expiration date of the lot/batch: 1 January 1994
- Stability under test conditions: stable
- Storage condition of test material: In the dark under nitrogen at 0 - 5°C
- Other:

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

21 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 19-24 mg/l

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

27 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Water quality

The water quality parameters were generally within the preferred ranges with the exception of the change in pH during the test. The pH change was a result of the good growth of the cultures and could not be avoided.

Analysis of aquatic toxicity test media

The reduction in Pentene-1 concentration during the test was = 20%.

Toxicity test

At nominal concentrations = 1.8% of a saturated solution, the mean measured concentration was less than the limit of determination of the analytical method. However, there was no inhibition of culture growth in these vessels and therefore toxicity data from these concentrations were not included in the calculation of the EC50 values.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72-hour ErC50 of the test substance to Raphidocelis subcapitata has been determined to be 21 mg/l.

Executive summary:

The toxicity of Pentene-1 to the unicellular alga Raphidocelis subcapitata has been determined in a 72 -hour growth inhibition test, adhering to OECD Guideline 201, without renewal of the test media.

The concentration of Pentene-1 in the test media was determined using a high performance liquid chromatography technique.

Concentrations decreased by less than 20% during the test. The toxicity data have been analysed on the basis of the geometric means of the concentrations measured at the start and end of the test.

The 72-hour ErC50 of the test substance to Raphidocelis subcapitata has been determined to be 21 mg/l. The 72 -hour NOEC has been determined to be 27 mg/l.

The test was conducted in a GLP facility in accordance with validated test guidelines therefore a reliability of 1 is assigned.