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Ecotoxicological information

Toxicity to microorganisms

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activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
equivalent or similar to guideline
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Version / remarks:
Method B-21
GLP compliance:
Specific details on test material used for the study:
Name: FAT 41001/F
Purity: >90%
Analytical monitoring:
not specified
not specified
Test organisms (species):
activated sludge
Test type:
Water media type:
Limit test:
Total exposure duration:
3 h
7.4 - 7.6
Dissolved oxygen:
4.7 - 5.1
Nominal and measured concentrations:
1, 3.2, 10, 32, 100 and 320 mg/L
Reference substance (positive control):
3 h
Dose descriptor:
Effect conc.:
> 320 mg/L
Nominal / measured:
Conc. based on:
test mat.
Results with reference substance (positive control):
3h IC50 for 3,5 - Dichlorophenol = 11 mg/L
Validity criteria fulfilled:
The IC50 of FAT 41001/F was found to be greater than 320 mg/L.
Executive summary:

A study was performed with FAT 41001/F to determine the toxicity to microorganisms. This test was carried out in accordance to OECD test guideline 209 and CIBA internal method B-21.

The concentration of test substance used: 1, 3.2, 10, 32, 100, 320 mg/L. 3,5 - Dichlorophenol was used as reference substance.

Based on the findings of the study, the 3h IC50 for FAT 21030/A was found to be greater than 320 mg/L. The 3h IC50 of reference substance was determined to be 11 mg/L.

Description of key information

3-hour IC50 of FAT 41001 to activated sludge micro-organisms was determined to be >320 mg/L. The most recent studies to evaluate the inhibitory effect of the test item FAT 41001 on the respiration rate of aerobic wastewater micro-organisms of activated sludge in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209 were performed in 1992. The 3-hour IC50 of FAT 41001 to activated sludge micro-organisms was determined to be >320 mg/L. Several other studies performed between 1982 to 1984 reported 24-hour IC50 to be >300 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
320 mg/L

Additional information