Trisodium 1-amino-4-[[3-[[4-chloro-6-[(sulphonatophenyl)amino]-1,3,5-triazin-2-yl]amino]-2,4,6-trimethyl-5-sulphonatophenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Version / remarks:

Method A-37

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 41001/F
Purity: >90 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

153 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

not specified

Parameter:

% degradation (DOC removal)

Value:

19

Sampling time:

28 d

Results with reference substance:

100% degradation was observed in 14 days.

Validity criteria fulfilled:

no

Interpretation of results:

not inherently biodegradable

Conclusions:

The inherent biodegradation of FAT 41001/F was observed to be 19 % in 28 days.

Executive summary:

Inherent biodegradablity of FAT 41001/F was determined according to OECD TG 302B (Zahn Wellens test) in DOC removal test. The test concentration was 153 mg/L DOC and test duration was 28 days. The bio-elimination of FAT 41001/F was determined to be 19 % after 28 days. Hence, FAT 41001/F is not inherently biodegradable under the conditions of this test.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Method A-22

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 41001/F
Purity: >90 %

Oxygen conditions:

not specified

Inoculum or test system:

not specified

Remarks on result:

not measured/tested

Details on results:

Not available

Parameter:

COD

Value:

905 mg O2/g test mat.

Validity criteria fulfilled:

no

Interpretation of results:

not readily biodegradable

Conclusions:

COD of FAT 41001/F was found to be 905 mg O2/g test mat.

Executive summary:

COD of FAT 41001/F was determined according to in house Ciba Geigy Method A22. Based on the findings of the study, the COD of test substance was found to be 905 mg O2/g. Considering the BOD5 value determined with the test substance 12 mg O2/g, the resulting BOD5/COD quotient is also 0.01, indicating that the test substance is unlikely to be biodegradable.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Method A-15

Version / remarks:

Comparable to international accepted guidelines.

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 41001/F
Purity: >90 %

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

5 d

Parameter followed for biodegradation estimation:

O2 consumption

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

12 mg O2/g test mat.

Validity criteria fulfilled:

no

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 of FAT 41001/F is 12 mg O2/g.

Executive summary:

BOD5 of FAT 41001/F was determined according to in house Ciba Geigy Methode A.15, which is comparable to internationally accepted guidelines. Based on these investigations, the BOD5 of test substance was found to be 12 mg O2/g. Considering the COD value determined with the test substance which is 905 mg O2/g, the resulting BOD5/COD quotient is 0.01, indicating that the test substance is not biodegradable.

Description of key information

The substance is a reactive dye and as such the substance should not be considered to be biodegradable. Key studies describe experimental techniques and results obtained in inherent biodegradation studies of FAT 41001/F. The most reliable study was performed according to OECD Guideline for testing chemicals No.302B, and biodegradation of FAT 41001/F after 28 d was 19 %, confirming that the substance should not be considered as readily biodegradable under the conditions of this test. In addition, determination of BOD and COD values and calculation of the BOD5/COD quotient resulted in a value close to zero. In additional supporting studies, the BOD/COD ratio was found to be value close to zero. Based on this estimation the substance is regarded to be not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed