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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48-h EC50 value is >100 mg/L in Daphnia magna.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

The acute toxicity of EEA-NH4 toDaphnia magnawas determined in a GLP-compliant study according to OECD guideline 202 (Kurume Laboratory, 2006c).

In this static limit test 20 daphnids (4 replicates of 5 animals) were exposed for 48 hours to 100 mg/L EEA-NH4 or a dilution water control (dechlorinated tap water). Immobility and other visble symptoms were observed at 24 and 48 hours after the start of exposure. The concentrations of test substance were analysed with high performance liquid chromatography (HPLC) at the start and end of exposure and determined at 99.0 and 99.3 percent of nominal concentration respectively.

No immobility or any other abnormal response was noticed in the test solution or control. Based on these findings, the 48-hour EC50 of EEA-NH4 forDaphnia magnawas determined to be >100 mg/L.